Flatt P R, Tan K S, Swanston-Flatt S K, Webster J D, Marks V
Comp Biochem Physiol A Comp Physiol. 1987;87(1):175-81. doi: 10.1016/0300-9629(87)90442-7.
The metabolic effects and secretory properties of a radiation-induced transplantable insulinoma were examined in 16-17 week old NEDH rats. Subcutaneous subscapular implantation of tumour fragments resulted in hyperphagia, increased body weight gain, marked hyperinsulinaemia and severe hypoglycaemia, with the resulting death of the recipient by 27 days. Ultimate tumour size was 2.1 +/- 0.4 g (mean +/- SEM). At 3 days after transplantation, plasma glucose and insulin responses to intraperitoneal glucose, insulin, arginine and adrenaline were similar to control rats. At 20 days, plasma glucose concentrations of insulinoma-bearing rats remained low throughout glucose tolerance tests, and insulin responsiveness to glucose stimulation was absent. 2-Deoxy-D-glucose produced only a small rise of glucose concentrations in tumour-bearing rats. Insulin sensitivity was not appreciably impaired at 20 days despite severe hyperinsulinaemia and hypoglycaemia. The ability of adrenaline and propranolol to suppress plasma insulin and raise plasma glucose concentrations was also retained. At 20 days, glucagon evoked a marked plasma insulin response with no change in plasma glucose concentrations. In contrast, arginine and glibenclamide failed to stimulate insulin above high basal concentrations.
在16 - 17周龄的NEDH大鼠中检测了辐射诱导的可移植胰岛素瘤的代谢效应和分泌特性。皮下肩胛下植入肿瘤碎片导致食欲亢进、体重增加、明显的高胰岛素血症和严重低血糖，受体在27天内死亡。最终肿瘤大小为2.1±0.4 g（平均值±标准误）。移植后3天，腹腔注射葡萄糖、胰岛素、精氨酸和肾上腺素后，血浆葡萄糖和胰岛素反应与对照大鼠相似。在20天时，患胰岛素瘤大鼠的血浆葡萄糖浓度在整个葡萄糖耐量试验中均保持较低水平，且对葡萄糖刺激无胰岛素反应。2 - 脱氧 - D - 葡萄糖在患瘤大鼠中仅使葡萄糖浓度有小幅升高。尽管存在严重的高胰岛素血症和低血糖，但在20天时胰岛素敏感性并未明显受损。肾上腺素和普萘洛尔抑制血浆胰岛素并升高血浆葡萄糖浓度的能力也得以保留。在20天时，胰高血糖素引起明显的血浆胰岛素反应，而血浆葡萄糖浓度无变化。相比之下，精氨酸和格列本脲未能使胰岛素高于高基础浓度水平。
