Flatt P R, Tan K S, Swanston-Flatt S K, Bailey C J, Marks V
Horm Res. 1987;27(1):47-52. doi: 10.1159/000180777.
Subcutaneous implantation of small fragments of a radiation-induced transplantable rat insulinoma into the subscapular region of 16- to 17-week-old male NEDH rats resulted, over a 22-day period, in the progressive development of marked hyperinsulinaemia and severe hypoglycaemia, despite a compensatory increase in food intake. Diurnal changes were examined at 3-hourly intervals for 24 h in control rats and tumour-bearing rats at 20-21 days after transplantation. The control animals exhibited distinct diurnal changes of food intake, glucose and insulin concentrations. Food intake was greatest between 17.00 and 23.00 h; plasma insulin was greatest between 20.00 and 23.00 h, and plasma glucose was raised at 20.00, 02.00 and 05.00 h, compared with the other times. In contrast, insulinoma-bearing rats displayed no diurnal changes other than a small decrease in food intake between 05.00 and 11.00 h. Plasma glucose and insulin concentrations were significantly different from control rats at all times, and food intake was significantly increased between 23.00 and 17.00 h. These observations demonstrate that the transplantable insulinoma not only causes hyperinsulinaemia and hypoglycaemia but results in hyperphagia and defective diurnal changes of food intake, plasma glucose and insulin concentrations. Interruption of nutrient intake by withdrawal of food for 6 h exacerbated the hypoglycaemia of insulinoma-bearing rats leading to coma.
将辐射诱导的可移植大鼠胰岛素瘤的小碎片皮下植入16至17周龄雄性NEDH大鼠的肩胛下区域，在22天的时间里，尽管食物摄入量有代偿性增加，但仍导致明显的高胰岛素血症和严重低血糖症的逐渐发展。在移植后20至21天，对对照大鼠和荷瘤大鼠每隔3小时进行24小时的昼夜变化检查。对照动物的食物摄入量、葡萄糖和胰岛素浓度呈现出明显的昼夜变化。食物摄入量在17:00至23:00之间最大；血浆胰岛素在20:00至23:00之间最大，与其他时间相比，血浆葡萄糖在20:00、02:00和05:00时升高。相比之下，荷胰岛素瘤大鼠除了在05:00至11:00之间食物摄入量略有下降外，没有表现出昼夜变化。血浆葡萄糖和胰岛素浓度在所有时间都与对照大鼠有显著差异，并且在23:00至17:00之间食物摄入量显著增加。这些观察结果表明，可移植的胰岛素瘤不仅会导致高胰岛素血症和低血糖症，还会导致食欲亢进以及食物摄入量、血浆葡萄糖和胰岛素浓度的昼夜变化缺陷。通过禁食6小时中断营养摄入会加剧荷胰岛素瘤大鼠的低血糖症，导致昏迷。
