Ladror Daniel, Pitt Bruce, Funk William
a Department of Preventive Medicine, Feinberg School of Medicine , Northwestern University , Chicago , IL , USA.
b Department of Environmental and Occupational Health, Graduate School of Public Health , University of Pittsburgh , Pittsburgh , PA , USA.
Biomarkers. 2018 Feb;23(1):44-50. doi: 10.1080/1354750X.2017.1375558. Epub 2017 Sep 18.
We present an ultra-sensitive, minimally-invasive method for quantifying cotinine in dried blood spot (DBS) samples as a biomarker of exposure to tobacco smoke that can be collected using a simple heel or finger prick to obtain blood samples.
Cotinine levels were measured in matched plasma and reconstituted DBS samples from smokers and nonsmokers to evaluate assay parameters. In addition, we applied this new method to finger-prick DBS samples that were collected from infants, children and young adults ages 1-21 to estimate exposure to tobacco smoke. Partitioning of cotinine across red blood cells and haematocrit effects were investigated.
Cotinine levels measured in matched plasma and reconstituted DBS samples from smokers and nonsmokers were found to be highly correlated (R=0.94), with 100% sensitivity and 94% specificity to differentiate reported smokers from nonsmokers. With this method, the LOQ is <0.25 ng/mL using a single 3.2 mm punch of a DBS, and haematocrit effects are negligible.
This sensitive, high-throughput and minimally-invasive method for quantifying cotinine in DBS samples provides a simple and cost effective means for estimating exposure to tobacco smoke in population based studies, and has particular advantages in studies involving infants and children.
我们提出一种超灵敏、微创的方法，用于定量检测干血斑（DBS）样本中的可替宁，作为接触烟草烟雾的生物标志物，该样本可通过简单的足跟或手指针刺采集血液样本获得。
测量吸烟者和非吸烟者匹配的血浆和重构DBS样本中的可替宁水平，以评估检测参数。此外，我们将这种新方法应用于从1至21岁的婴儿、儿童和年轻人采集的手指针刺DBS样本，以估计烟草烟雾暴露情况。研究了可替宁在红细胞中的分配情况和血细胞比容的影响。
发现吸烟者和非吸烟者匹配的血浆和重构DBS样本中测得的可替宁水平高度相关（R=0.94），区分报告的吸烟者和非吸烟者的灵敏度为100%，特异性为94%。使用这种方法，用单个3.2毫米的DBS冲孔，定量下限<0.25 ng/mL，血细胞比容的影响可忽略不计。
这种用于定量检测DBS样本中可替宁的灵敏、高通量和微创方法，为基于人群的研究中估计烟草烟雾暴露提供了一种简单且经济有效的手段，在涉及婴儿和儿童的研究中具有特别的优势。
