Stereoselective Analysis and Degradation of Pyrisoxazole in Cabbage, Pakchoi, and Pepper by Liquid Chromatography Tandem Mass Spectrometry.

Pyrisoxazole is a chiral fungicide with high sterilizing activity to the plant pathogenic bacteria and thus can be used for protecting the vegetables from gray mold, powdery mildew, and brown rot. The present work aimed to explore its stereoselective degradation in cabbage, pakchoi, and pepper samples. The enantioseparation and analysis on chiral column Lux Cellulose-3 based on liquid chromatography tandem mass spectrometry was developed coupled to the QuEChERS method. The recoveries of the stereoisomers in various vegetables ranged from 72.6 to 124% with RSD lower than 5.0%. Enantioselective dissipation of pyrisoxazole in vegetables displayed that (-)-A-pyrisoxazole was preferentially degraded versus (+)-A-pyrisoxazole in all the vegetables. (+)-B-pyrisoxazole was preferentially degraded in cabbage, while there was no obvious enantioselectivity in pakchoi and pepper. Meanwhile, stereoselectivity analysis demonstrated that (±)-A-pyrisoxazole was degraded faster than (±)-B-pyrisoxazole in pakchoi and pepper, while there was no stereoselective degradation in cabbages.