State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection , Chinese Academy of Agricultural Sciences , Beijing 100193 , P. R. China.
J Agric Food Chem. 2019 Sep 11;67(36):10256-10264. doi: 10.1021/acs.jafc.9b03607. Epub 2019 Aug 30.
In this study, a simple and effective chiral analytical method was developed to monitor prothioconazole and prothioconazole-desthio at the enantiomeric level using supercritical fluid chromatography-tandem triple quadrupole mass spectrometry. The baseline enantioseparation for prothioconazole and prothioconazole-desthio was achieved within 2 min on a Chiralcel OD-3 column with CO/0.2% acetic acid-5 mmol/L ammonium acetate 2-propanol (85:15, v/v) as the mobile phase at a flow rate of 1.5 mL/min and column temperature of 25 °C. The limit of quantitation for each enantiomer was 5 μg/kg, with a baseline resolution of >3.0. The results of enantioselective dissipation showed that -(-)-prothioconazole was preferentially degraded in tomato, cucumber, and pepper under greenhouse conditions. -(-)-prothioconazole-desthio was preferentially degraded in tomato and cucumber; however, -(+)-prothioconazole-desthio was preferentially degraded in pepper. Results of this study may help to facilitate more accurate risk assessment of prothioconazole and its major metabolite in agricultural products.
在这项研究中，开发了一种简单有效的手性分析方法，使用超临界流体色谱-串联三重四极杆质谱法在对映体水平上监测丙硫菌唑和丙硫菌唑脱硫。在 Chiralcel OD-3 柱上，以 CO/0.2% 乙酸-5 mmol/L 乙酸铵 2-丙醇（85:15，v/v）为流动相，流速为 1.5 mL/min，柱温为 25°C，在 2 min 内即可实现丙硫菌唑和丙硫菌唑脱硫的基线对映体分离。每个对映异构体的定量限均为 5 μg/kg，基线分辨率>3.0。对映选择性消解的结果表明，在温室条件下，-(-)-丙硫菌唑优先在番茄、黄瓜和辣椒中降解。-(-)-丙硫菌唑脱硫优先在番茄和黄瓜中降解；然而，-(+)-丙硫菌唑脱硫优先在辣椒中降解。本研究结果可能有助于更准确地评估丙硫菌唑及其在农产品中的主要代谢物的风险。
