开发并验证一种液相色谱-串联质谱法，用于同时定量测定 5 种唑类抗真菌药物和 1 种活性代谢物。
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite.
机构信息
Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, United States.
Department of Laboratory Medicine, Cleveland Clinic, Cleveland, OH, United States.
出版信息
Clin Chim Acta. 2017 Nov;474:8-13. doi: 10.1016/j.cca.2017.08.032. Epub 2017 Aug 31.
BACKGROUND
Azole antifungal medications are often administered to prevent or treat invasive fungal infections. These infections are deadly in the immunocompromised population. Therapeutic drug monitoring of the azole antifungal medications may potentially decrease morbidity and mortality in patients undergoing azole treatment.
METHODS
To assist with azole therapeutic drug monitoring, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for 6 azole analytes: fluconazole, voriconazole, posaconazole, isavuconazole, itraconazole, and its active metabolite hydroxyitraconazole.
RESULTS
The validated method solely required a protein precipitation step before subsequent dilution and injection onto the LC-MS/MS system. Furthermore, the analysis time was <2min per sample.
CONCLUSIONS
This method enables measurement of all 6 of these analytes into a single LC-MS/MS assay.
背景
唑类抗真菌药物常用于预防或治疗侵袭性真菌感染。在免疫功能低下的人群中，这些感染是致命的。唑类抗真菌药物的治疗药物监测可能会降低接受唑类药物治疗的患者的发病率和死亡率。
方法
为了协助唑类药物的治疗药物监测，开发并验证了一种液相色谱串联质谱（LC-MS/MS）方法，用于检测 6 种唑类分析物：氟康唑、伏立康唑、泊沙康唑、伊曲康唑、其活性代谢物羟基伊曲康唑。
结果
该验证方法仅在随后稀释和注入 LC-MS/MS 系统之前需要进行蛋白沉淀步骤。此外，每个样品的分析时间<2 分钟。
结论
该方法能够将这 6 种分析物全部测量到单个 LC-MS/MS 测定中。
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