Department of Biochemistry and Molecular Biology, University of Chicago, United States.
Eli and Edythe Broad Center for Regenerative Medicine, UCLA, United States; The Molecular Biology Institute, UCLA, United States; The Jonsson Comprehensive Cancer Center, UCLA, United States; Department of Dermatology, David Geffen School of Medicine, UCLA, United States.
Methods. 2018 Jan 15;133:44-53. doi: 10.1016/j.ymeth.2017.08.018. Epub 2017 Aug 31.
Neural progenitor cells hold significant promise in a variety of clinical settings. While both the brain and spinal cord harbor endogenous neural progenitor or stem cells, they typically are not capable of repopulating neural populations in case of injury or degenerative disease. In vitro systems for the culture of neural progenitors has come a long ways due to advances in the method development. Recently, many groups have shown that manipulation of the oxygen-sensing pathway leading to activation of hypoxia inducible factors (HIFs) that can influence the proliferation, differentiation or maturation of neural progenitors. Moreover, different oxygen concentrations appear to affect lineage specification of neural progenitors upon their differentiation in vitro. Here we summarize some of these studies in an attempt to direct effort towards implementation of best methods to advance the use of neural progenitors from basic development towards clinical application.
神经祖细胞在多种临床环境中具有重要的应用前景。虽然大脑和脊髓中都存在内源性神经祖细胞或干细胞，但它们通常不能在受伤或退行性疾病的情况下重新填充神经群体。由于方法学的发展，体外培养神经祖细胞的系统已经取得了很大的进展。最近，许多研究小组已经表明，对氧感应途径的操纵可以激活缺氧诱导因子（HIFs），从而影响神经祖细胞的增殖、分化或成熟。此外，不同的氧浓度似乎会影响体外分化过程中神经祖细胞的谱系特化。在这里，我们总结了其中的一些研究，试图指导人们努力实施最佳方法，以促进神经祖细胞从基础发育到临床应用的应用。
