Univ Lyon, Université Claude Bernard Lyon 1, Inserm, Stem Cell and Brain Research Institute U1208, Bron, France.
Neurogenetics Department, Ecole Pratique des Hautes Etudes, PSL Research University, Paris, France.
Stem Cells. 2019 Nov;37(11):1381-1388. doi: 10.1002/stem.3071. Epub 2019 Aug 20.
Development of the forebrain occurs in a stepwise manner from a pool of neural progenitors (NPs), which differs over space and time to produce distinct progenies. The sequence of events leading to the generation of the exquisite complexity of cell types that compose this tissue has been described in great detail at the population level. Recent advances in histology and transcriptomics have allowed probing spatial and temporal heterogeneity and dynamics of NPs at the single-cell level. Clonal fate mapping studies highlight a deterministic behavior as well as the existence of trajectories in the lineage progression of prenatal and postnatal NPs, whereas single-cell transcriptomic studies shed new light on the transcriptional signatures of these processes. Here, we review this recent work and integrate it to our current understanding of forebrain germinal activity at prenatal and postnatal time points. Stem Cells 2019;37:1381-1388.
大脑前脑的发育是一个逐步的过程，从神经祖细胞（NPs）池开始，这个过程在空间和时间上有所不同，产生不同的祖细胞。在群体水平上，已经详细描述了导致组成该组织的细胞类型的精细复杂性产生的一系列事件。组织学和转录组学的最新进展允许在单细胞水平上探测 NPs 的空间和时间异质性和动态。克隆命运映射研究强调了确定性行为以及产前和产后 NPs 谱系进展中的轨迹的存在，而单细胞转录组学研究则揭示了这些过程的转录特征。在这里，我们回顾了这项最近的工作，并将其整合到我们目前对产前和产后时间点大脑前脑生殖活动的理解中。干细胞 2019;37:1381-1388.
