Khan S A, Söder O, Syed V, Gustafsson K, Lindh M, Ritzén E M
Int J Androl. 1987 Apr;10(2):495-503. doi: 10.1111/j.1365-2605.1987.tb00224.x.
Homogenates of whole testis, isolated seminiferous tubules, testicular cytosol, conditioned media from seminiferous tubules obtained from intact or cryptorchid rats, as well as seminiferous tubules devoid of peritubular cells, showed high concentrations of interleukin-1 (IL-1). Cytosol from spleen showed low IL-1 activity, while no activity was found in cytosol from heart, kidney, prostate, ovary or liver. Interleukin-1 activity was not detected in spent medium from cultures of immature Sertoli cells (10-day-old rats) or from peritubular cells or in homogenates of interstitial cells from adult rats. Ultrogel AcA 44 gel chromatography and HPLC size exclusion chromatography exhibited a single peak of IL-1 activity corresponding to a relative molecular mass of 17,000-20,000 (Mr = 17-20 K). Similarly, chromatofocusing revealed only one peak of activity with an apparent isoelectric point of 5-6. It is concluded that the rat testis contains large amounts of an IL-1 alpha-like factor. The adult Sertoli cell or possibly germ cells are suggested as its primary source. Testicular IL-1-like activity is of particular interest in view of the intense cell proliferation during spermatogenesis, and the tendency to testicular relapse of acute lymphoblastic leukaemia.
完整睾丸、分离的生精小管、睾丸胞质溶胶、来自完整或隐睾大鼠的生精小管的条件培养基，以及缺乏管周细胞的生精小管的匀浆，均显示出高浓度的白细胞介素-1（IL-1）。脾脏胞质溶胶显示出低IL-1活性，而在心脏、肾脏、前列腺、卵巢或肝脏的胞质溶胶中未发现活性。在未成熟支持细胞（10日龄大鼠）培养物的用过的培养基中、管周细胞培养物中或成年大鼠间质细胞的匀浆中均未检测到白细胞介素-1活性。Ultrogel AcA 44凝胶过滤色谱法和HPLC尺寸排阻色谱法显示IL-1活性的单峰，对应相对分子质量为17,000 - 20,000（Mr = 17 - 20K）。同样，色谱聚焦仅显示一个活性峰，表观等电点为5 - 6。结论是大鼠睾丸含有大量的IL-1α样因子。推测成年支持细胞或可能的生殖细胞是其主要来源。鉴于生精过程中强烈的细胞增殖以及急性淋巴细胞白血病睾丸复发的倾向，睾丸IL-1样活性特别令人关注。
