戈谢病。影响培养的皮肤成纤维细胞4-甲基伞形酮基-β-D-葡萄糖苷酶活性的因素。
Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts.
作者信息
Butterworth J, Broadhead D M
出版信息
Clin Genet. 1978 Aug;14(2):77-9. doi: 10.1111/j.1399-0004.1978.tb02109.x.
Abstract
Phosphate-buffered saline was found to inhibit acid beta-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.
摘要
发现磷酸盐缓冲盐水可抑制培养细胞的酸性β-葡萄糖苷酶活性。这种作用归因于磷酸盐缓冲盐水的pH值和氯化物含量。用蔗糖（0.25M）洗涤细胞以去除残留的磷酸盐缓冲盐水可有效消除这种抑制作用。
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