血小板活化因子（PAF）补充对冷冻保存的公牛精子（人工授精用）ATP含量的影响。

Effects of the platelet-activating factor (PAF) supplementation on ATP content of cryopreserved bull spermatozoa (AI).

作者信息

Lecewicz M, Kordan W, Kamiński S, Majewska A M, Strzeżek R

机构信息

出版信息

Pol J Vet Sci. 2017 Mar 1;20(2):421-423. doi: 10.1515/pjvs-2017-0051.

DOI:10.1515/pjvs-2017-0051

PMID:28865217

Abstract

The aim of this study was to investigate the effect of PAF supplementation in semen extender on ATP content in cryopreserved bull spermatozoa used for artificial insemination at different time intervals. Cryopreserved semen was treated with different concentrations of PAF: 1×10-5M, 1×10-6M, 1×10-7M, 1×10-8M and 1×10-9M at 37°C. In the present work we showed that content of ATP in cryopreserved semen supplemented with 1×10-9M PAF was statistically significantly higher at 90 and 120 minutes of incubation in comparison to the control group (p≤0.05). Present study indicates the potential influence of PAF on ATP content in male spermatozoa via it's protective role towards mitochondria metabolic activity.

摘要

本研究的目的是调查在精液稀释剂中添加血小板活化因子（PAF）对用于人工授精的冷冻公牛精子在不同时间间隔下ATP含量的影响。将冷冻精液在37°C下用不同浓度的PAF处理：1×10-5M、1×10-6M、1×10-7M、1×10-8M和1×10-9M。在本研究中，我们发现，与对照组相比，添加1×10-9M PAF的冷冻精液在孵育90分钟和120分钟时，ATP含量在统计学上显著更高（p≤0.05）。本研究表明PAF通过对线粒体代谢活性的保护作用，对雄性精子中的ATP含量具有潜在影响。