Biofilm Centre, Aquatische Biotechnologie, Universität Duisburg-Essen, Universitätsstraße 5, 45141 Essen, Germany.
Department of Chemistry/Nanochemistry Research Institute, Curtin University, Bentley, WA 6845, Australia.
Colloids Surf B Biointerfaces. 2017 Nov 1;159:639-643. doi: 10.1016/j.colsurfb.2017.08.023. Epub 2017 Aug 31.
Microorganisms adhere to surfaces and, subsequently, form biofilms. This process is of major interest in biotechnology, environmental sciences and medicine. It is crucial to understand the mechanisms of interactions between substratum and cells or biofilms. By combining force mapping-based atomic force microscopy (AFM) with pyrite-modified cantilevers we quantified the adhesion forces between undenatured planktonic or biofilm cells of Sulfobacillus thermosulfidooxidans and the substratum pyrite with values of 2.6±0.3nN and 77.3±7.1pN, respectively. This was achieved under natural conditions without any artefact resulting from the use of denaturing chemicals such as glutaraldehyde. This new technique is unique for quantifying the real interaction forces between cells or biofilms and their substrata.
微生物附着在表面上，随后形成生物膜。这个过程在生物技术、环境科学和医学中非常重要。了解基质与细胞或生物膜之间相互作用的机制至关重要。我们通过将基于力映射的原子力显微镜 (AFM) 与黄铁矿修饰的微悬臂梁相结合，在自然条件下，无需使用戊二醛等变性化学物质产生任何人为影响，定量测量了未经变性的浮游生物或 Sulfobacillus thermosulfidooxidans 生物膜细胞与基质黄铁矿之间的粘附力，分别为 2.6±0.3nN 和 77.3±7.1pN。这项新技术独特之处在于能够定量测量细胞或生物膜与其基质之间的真实相互作用力。
