Centro Tecnológico de la Carne de Galicia, Adva. Galicia n° 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.
Department of Food Engineering, Faculty of Animal Science and Food Engineering, University of São Paulo, 225 Duque de Caxias Norte Ave, Jardim Elite, 13.635-900 Pirassununga, São Paulo, Brazil.
Food Res Int. 2017 Sep;99(Pt 3):979-985. doi: 10.1016/j.foodres.2017.03.043. Epub 2017 Mar 24.
The phenolic compounds of extracts from Ascophyllum nodosum (ANE), Bifurcaria bifurcata (BBE) and Fucus vesiculosus (FVE) from Galicia (NW Spain) were analyzed by liquid chromatography-diode array detection coupled to negative electrospray ionization-tandem mass spectrometry (LC-DAD-ESI-MS/MS) with the interest to evaluate their potential application as functional ingredients. Phlorotannins were tentatively identified as the main phenolic compounds in the three extracts, followed by phenolic acids, and flavonoids. Fuhalols were present in ANE and BBE, while hydroxyfuhalols were identified in BBE and FVE. Eckol derivatives were present in the three extracts. Quinic acid derivatives were tentatively identified in the three seaweed species; in addition, ANE showed specifically hydroxybenzoic and rosmarinic acid derivatives, BBE showed rosmarinic acid, and FVE contained p-coumaric and ferulic acid derivatives. Regarding flavonoids, acacetin derivatives were tentatively identified in the three extracts, hispidulin and a gallocatechin derivative were specifically detected in ANE, and cypellocarpin C was present in BBE. In conclusion, all brown seaweed extracts studied could be exploited as sources of antioxidant phenolic compounds with potential applications in the food and health sectors.
采用液相色谱-二极管阵列检测-电喷雾串联质谱联用技术（LC-DAD-ESI-MS/MS）分析了来自西班牙西北部加利西亚的泡叶藻（ANE）、叉珊藻（BBE）和掌状海带（FVE）提取物中的酚类化合物，旨在评估其作为功能性成分的潜在应用。三种提取物中的主要酚类化合物被推测为岩藻黄质，其次为酚酸和类黄酮。ANE 和 BBE 中存在富勒醇，而 BBE 和 FVE 中则存在羟基富勒醇。三种提取物中均存在 Eckol 衍生物。三种海藻中均推测存在奎宁酸衍生物；此外，ANE 中还特异性地存在羟基苯甲酸和迷迭香酸衍生物，BBE 中存在迷迭香酸，FVE 中则含有对香豆酸和阿魏酸衍生物。关于类黄酮，三种提取物中均推测存在白杨素衍生物，ANE 中特异性地检测到金丝桃苷和没食子儿茶素衍生物，而 BBE 中则存在 Cypellocarpin C。总之，研究中所有的褐藻提取物都可以作为抗氧化酚类化合物的来源，具有在食品和健康领域应用的潜力。
