Rood Ineke G H, Li Qingge
State Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
State Key Laboratory of Cellular Stress Biology, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Engineering Research Center of Molecular Diagnostics of the Ministry of Education, School of Life Sciences, Xiamen University, Xiamen, Fujian, China.
Diagn Microbiol Infect Dis. 2017 Nov;89(3):245-250. doi: 10.1016/j.diagmicrobio.2017.07.013. Epub 2017 Aug 1.
Enterobacteriaceae are a common source of both community- and hospital-acquired infections. The resistance of Enterobacteriaceae to β-lactam antibiotics is a major public health problem and rapid detection is essential for the proper management of infections. Not only for the initiation of the proper antimicrobial regimen but also to stop the spread of multi-resistant bacteria. In this review, molecular methods that detect extended-spectrum-β-lactamase-harboring and carbapenem-resistant Enterobacteriaceae were discussed. More specifically the performances of assays that were tested in a clinical situation were explored. When compared with conventional phenotypical methods (the gold standard) all molecular methods generated results faster and showed a higher sensitivity. Because molecular methods only detect selected genes, it is important to have good knowledge on the local gene pool. Furthermore, result need to be interpreted within a defined epidemiological context. Therefore, at this moment molecular methods should be seen as an important tool to complement rather than replace conventional methods.
肠杆菌科细菌是社区获得性感染和医院获得性感染的常见来源。肠杆菌科细菌对β-内酰胺类抗生素的耐药性是一个重大的公共卫生问题，快速检测对于感染的合理管理至关重要。这不仅关乎启动恰当的抗菌治疗方案，还关乎阻止多重耐药菌的传播。在本综述中，讨论了检测产超广谱β-内酰胺酶和耐碳青霉烯类肠杆菌科细菌的分子方法。更具体地说，探讨了在临床环境中进行测试的检测方法的性能。与传统的表型方法（金标准）相比，所有分子方法产生结果的速度更快，且灵敏度更高。由于分子方法只能检测特定基因，因此深入了解当地的基因库非常重要。此外，结果需要在特定的流行病学背景下进行解读。因此，目前分子方法应被视为一种重要的补充工具，而非替代传统方法。
