Aksaray University, Technical Vocational School, Department of Chemistry Technology, 68100, Aksaray, Turkey.
Aksaray University, Faculty of Science and Letters, Department of Biotechnology and Molecular Biology, 68100 Aksaray, Turkey.
Int J Biol Macromol. 2017 Dec;105(Pt 2):1401-1411. doi: 10.1016/j.ijbiomac.2017.08.161. Epub 2017 Sep 1.
Practical applications of biopolymers in different industries are gaining considerable increase day by day. But still, these biopolymers lack important properties in order to meet the industrial demands. In the same regard, in the current study, chitosan composite films are produced by incorporating diatomite soil at two different concentrations. In order to obtain a homogeneous film, glutaraldehyde was supplemented to chitosan solution as a cross-linker. Compositing diatomaceous earth to chitosan film resulted in improvement of various important physicochemical properties compared to control such as; enhanced film wettability, increase elongation at break and improved thermal stability (264-277°C). The microstructure of the film was observed to haveconsisted of homogeneously distributed blister-shaped structures arised due to the incorporation of diatomite. The incorporation of diatomite did not influence the overall antioxidant activity of the composite films, which can be ascribe to the difficulty radicals formation. Chitosan film incorporated with increasing fraction of diatomite revealed a notable enhancement in the antimicrobial activity. Additionally with the present study, for the first time possible interactions between chitosan/diatomite were determined via quantum chemical calculations. Current study will be helpful in giving a new biotechnological perspective to diatom in terms of its successful application in hydrophobic composite film production.
生物聚合物在不同行业中的实际应用正日益得到广泛应用。但这些生物聚合物仍然缺乏一些重要的特性，无法满足工业需求。在这方面，本研究通过在两种不同浓度下掺入硅藻土来生产壳聚糖复合膜。为了获得均匀的薄膜，戊二醛作为交联剂被添加到壳聚糖溶液中。与对照相比，将硅藻土复合到壳聚糖薄膜中可改善各种重要的物理化学性质，例如提高了薄膜的润湿性、断裂伸长率和热稳定性（264-277°C）。观察到薄膜的微观结构由均匀分布的泡状结构组成，这是由于硅藻土的掺入而产生的。硅藻土的掺入并没有影响复合薄膜的整体抗氧化活性，这可以归因于自由基形成的困难。壳聚糖薄膜中硅藻土含量的增加显著提高了其抗菌活性。此外，通过本研究，首次通过量子化学计算确定了壳聚糖/硅藻土之间的可能相互作用。本研究将有助于从生物科技的角度对硅藻进行新的应用，使其成功应用于疏水性复合膜的生产。
