College of Food Science and Engineering, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
College of Food Science and Engineering, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China.
Int J Biol Macromol. 2019 Jul 15;133:51-57. doi: 10.1016/j.ijbiomac.2019.04.067. Epub 2019 Apr 11.
Active films based on chitosan incorporated Herba Lophatheri extract (HLE) with different concentrations were developed. Physicochemical properties of the chitosan films incorporated HLE, including density, opacity, moisture content, color, water solubility, swelling, water vapor permeability and oil resistance were measured. Biological activities of the films include antioxidant activity and antimicrobial properties, which were characterized in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Oxford cup method, respectively. The potential interactions between chitosan and HLE in the films were investigated by Attenuated total reflectance-Fourier transform infrared analysis and X-ray diffraction. The results indicated that the moisture content, water solubility, swelling degree, water vapor permeability and oil absorption rate of chitosan/HLE films decreased up to 14.81%, 38.97%, 48.03%, 69.23% and 80% in comparison with the control chitosan film. The DPPH free radical scavenging activity of chitosan/HLE films increased by nearly 3.5 folds, and the diameter of inhibitory zone of the chitosan/HLE films extract solution against Staphylococcus aureus and Escherichia coli increased 17.02% and 19.28%, respectively. Additionally, the incorporation of HLE caused interactions between chitosan and HLE and gave rise to the chitosan/HLE films more opacity, darker, redness and yellowness appearance. In summary, the addition of HLE enhanced the moisture and oil resistance, antioxidant activity which declined with time, and antimicrobial activity of the chitosan film, which were all desirable for food packaging.
基于壳聚糖的活性薄膜，其中掺入了不同浓度的夏枯草提取物（HLE）。测量了掺入 HLE 的壳聚糖薄膜的物理化学性质，包括密度、不透明度、水分含量、颜色、水溶性、溶胀、水蒸气透过率和耐油性。薄膜的生物活性包括抗氧化活性和抗菌性能，分别通过 2,2-二苯基-1-苦基肼（DPPH）自由基清除活性和牛津杯法进行表征。通过衰减全反射-傅里叶变换红外分析和 X 射线衍射研究了薄膜中壳聚糖和 HLE 之间的潜在相互作用。结果表明，与对照壳聚糖膜相比，壳聚糖/HLE 膜的水分含量、水溶性、溶胀度、水蒸气透过率和吸油率分别降低了 14.81%、38.97%、48.03%、69.23%和 80%。壳聚糖/HLE 薄膜的 DPPH 自由基清除活性增加了近 3.5 倍，壳聚糖/HLE 薄膜提取物溶液对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别增加了 17.02%和 19.28%。此外，HLE 的掺入导致了壳聚糖和 HLE 之间的相互作用，使壳聚糖/HLE 薄膜的不透明度、颜色更深、更红、更黄。总之，HLE 的添加增强了壳聚糖膜的耐湿性和耐油性、抗氧化活性（随时间降低）和抗菌活性，这对食品包装都是理想的。
