Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
Henan Provincial Key Laboratory of Functional Textile Materials, Zhongyuan University of Technology, Zhengzhou 450007, China; Collaborative Innovation Center of Textile and Garment Industry, Henan Province, Zhengzhou 450007, China.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:232-242. doi: 10.1016/j.msec.2017.05.133. Epub 2017 May 22.
To better mimic natural bone, a graphene oxide-hydroxyapatite/silk fibroin (cGO-HA/SF) scaffold was fabricated by biomineralizing carboxylated GO sheets, blending with SF, and freeze-drying. The material has increasing porosity and decreasing density from outside to inside. Analysis of GO mineralization in simulated body fluid indicated that carboxylation and Chitosan may synergistically regulate HA growth along the c-axis of weakly crystalline, rod-like GO-HA particles. Compared with HA/SF gradient composites, a cGO-HA gradient scaffold with cGO:HA mass ratio 1:4 has 5-fold and 2.5-fold higher compressive strength and compressive modulus, respectively. Additionally, the cGO-HA/SF composite stimulated mouse mesenchymal stem cell adhesion and proliferation, alkaline phosphatase secretion, and mineral deposition more strongly than HA/SF and pure HA scaffolds. Hence, the material may prove to be an excellent and versatile scaffold for bone tissue engineering.
为了更好地模拟天然骨，通过矿化羧基化 GO 片、与丝素蛋白（SF）混合以及冷冻干燥，制备了氧化石墨烯-羟基磷灰石/丝素蛋白（cGO-HA/SF）支架。该材料的孔隙率从外向内逐渐增加，密度逐渐降低。在模拟体液中的 GO 矿化分析表明，羧化和壳聚糖可能协同调节沿弱晶、棒状 GO-HA 颗粒的 c 轴生长的 HA。与 HA/SF 梯度复合材料相比，cGO:HA 质量比为 1:4 的 cGO-HA 梯度支架的抗压强度和抗压模量分别提高了 5 倍和 2.5 倍。此外，cGO-HA/SF 复合材料对小鼠间充质干细胞的黏附、增殖、碱性磷酸酶分泌和矿化沉积的刺激作用强于 HA/SF 和纯 HA 支架。因此，该材料可能被证明是一种用于骨组织工程的优秀且多功能的支架。
