组蛋白mRNA的产生与降解
Birth and Death of Histone mRNAs.
作者信息
Marzluff William F, Koreski Kaitlin P
机构信息
Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Integrative Program for Biological and Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Curriculum in Genetics and Molecular Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
出版信息
Trends Genet. 2017 Oct;33(10):745-759. doi: 10.1016/j.tig.2017.07.014. Epub 2017 Aug 31.
In metazoans, histone mRNAs are not polyadenylated but end in a conserved stem-loop. Stem-loop binding protein (SLBP) binds to the stem-loop and is required for all steps in histone mRNA metabolism. The genes for the five histone proteins are linked. A histone locus body (HLB) forms at each histone gene locus. It contains factors essential for transcription and processing of histone mRNAs, and couples transcription and processing. The active form of U7 snRNP contains the HLB component FLASH (FLICE-associated huge protein), the histone cleavage complex (HCC), and a subset of polyadenylation factors including the endonuclease CPSF73. Histone mRNAs are rapidly degraded when DNA replication is inhibited by a 3' to 5' pathway that requires extensive uridylation of mRNA decay intermediates.
在多细胞动物中，组蛋白mRNA不进行多聚腺苷酸化，而是以一个保守的茎环结构结尾。茎环结合蛋白（SLBP）与茎环结合，是组蛋白mRNA代谢所有步骤所必需的。五种组蛋白的基因是连锁的。在每个组蛋白基因位点会形成一个组蛋白基因位点体（HLB）。它包含组蛋白mRNA转录和加工所必需的因子，并将转录和加工过程联系起来。U7 snRNP的活性形式包含HLB组分FLASH（FLICE相关巨蛋白）、组蛋白切割复合体（HCC）以及包括核酸内切酶CPSF73在内的多聚腺苷酸化因子的一个子集。当DNA复制通过一种3'至5'途径被抑制时，组蛋白mRNA会迅速降解，该途径需要mRNA降解中间体进行广泛的尿苷酸化。
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