Y3**非编码RNA促进组蛋白mRNA的3'末端加工。
The Y3** ncRNA promotes the 3' end processing of histone mRNAs.
作者信息
Köhn Marcel, Ihling Christian, Sinz Andrea, Krohn Knut, Hüttelmaier Stefan
机构信息
Institute of Molecular Medicine, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
Institute of Pharmacy, Martin-Luther-University Halle-Wittenberg, Saxony-Anhalt 06120, Germany;
出版信息
Genes Dev. 2015 Oct 1;29(19):1998-2003. doi: 10.1101/gad.266486.115.
We demonstrate that the Y3/Y3** noncoding RNAs (ncRNAs) bind to the CPSF (cleavage and polyadenylation specificity factor) and that Y3** associates with the 3' untranslated region (UTR) of histone pre-mRNAs. The depletion of Y3** impairs the 3' end processing of histone pre-mRNAs as well as the formation and protein dynamics of histone locus bodies (HLBs), the site of histone mRNA synthesis and processing. HLB morphology is also disturbed by knockdown of the CPSF but not the U7-snRNP components. In conclusion, we propose that the Y3** ncRNA promotes the 3' end processing of histone pre-mRNAs by enhancing the recruitment of the CPSF to histone pre-mRNAs at HLBs.
我们证明Y3/Y3非编码RNA（ncRNA）与切割及聚腺苷酸化特异性因子（CPSF）结合，且Y3与组蛋白前体mRNA的3'非翻译区（UTR）相关联。Y3的缺失会损害组蛋白前体mRNA的3'末端加工以及组蛋白基因座体（HLB）的形成和蛋白质动态变化，HLB是组蛋白mRNA合成和加工的场所。CPSF的敲低会扰乱HLB形态，但U7小核核糖核蛋白（U7-snRNP）组分的敲低则不会。总之，我们提出Y3 ncRNA通过增强CPSF在HLB处募集到组蛋白前体mRNA来促进组蛋白前体mRNA的3'末端加工。
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