Laboratory of Biochemistry and Molecular Biology, Code UR13ES34 Research Unit, Carthage University, Faculty of Sciences of Bizerte, Zarzouna, Bizerte, Tunisia.
Laboratory of Genetics, Faculty of Sciences of Bizerte, 7021 Zarzouna, Tunisia.
Microb Pathog. 2017 Oct;111:225-231. doi: 10.1016/j.micpath.2017.08.049. Epub 2017 Sep 1.
In the current study, bacterial diversity was investigated in root nodules of Sulla pallida and Sulla capitata. The isolates were analyzed on the basis of their phenotypic and molecular characteristics. The phylogenetic analysis based on 16S rRNA and housekeeping genes (recA and atpD) showed that the isolated bacteria related to Sinorhizobium, Neorhizobium, Phyllobacterium, Arthrobacter, Variovorax and Pseudomonas genera. This is the first report of Neorhizobium genus associated with Hedysarum genus. Phenotypically, all strains tolerate the elevated temperature of 40 °C, and salt stress at a concentration of 2%. In addition, the isolates failed to induce nodulation on their original host; and the symbiotic genes could not be amplified, suggesting that these strains are endophytic bacteria.
在本研究中，对 Sulla pallida 和 Sulla capitata 根瘤中的细菌多样性进行了调查。根据表型和分子特征对分离株进行了分析。基于 16S rRNA 和看家基因（recA 和 atpD）的系统发育分析表明，分离出的细菌与 Sinorhizobium、Neorhizobium、Phyllobacterium、Arthrobacter、Variovorax 和 Pseudomonas 属有关。这是首次报道与荆豆属相关的 Neorhizobium 属。表型上，所有菌株都能耐受 40°C 的高温和 2%的盐胁迫。此外，这些分离株不能在其原始宿主上诱导结瘤，并且共生基因也不能扩增，这表明这些菌株是内生细菌。
