Shangrao Normal University, College of Life Science, Shangrao, Jiangxi, China.
Xinjiang Academy of Environmental Protection Science, Xinjiang Key Laboratory for Environmental Pollution Monitoring and Risk Warning, Urumqi, Xinjiang, China.
PeerJ. 2023 Aug 28;11:e15934. doi: 10.7717/peerj.15934. eCollection 2023.
The bacterial diversity in stem storage liquid samples grown in Shaya County and Yuli County of the Tarim River Basin was investigated. A culture-dependent (dilution spread plate method) and culture-independent method (PCR-RFLP technique) were used to identify the endophytic bacteria community structure and composition in in Tarim River Basin. Sixty-six bacterial strains were isolated from stem storage liquid samples on three agar media. The 16S rDNA gene was amplified and sequenced using bacterial universal primers. Phylogenetic analysis showed that the 66 strains belonged to three phyla (Firmicutes, Actinomycetes, and Gamma-Proteobacteria) and included 16 genera and 29 species. Among them, (27.27%) and (19.69%) were the dominant isolates. CGM-17 was a potentially new species of . Restriction fragment length polymorphism of 16S rDNA gene amplified by polymerase chain reaction (PCR-RFLP) revealed 48 operational taxonomic units (OTUs). Phylogenetic analysis indicated that the 48 OTUs belonged to Firmicutes, Actinobacteria, Proteobacteria (α-, β-, γ-subgroup), Bacteroidetes, and Verrucomicrobia. Gamma-Proteobacteria was the dominant group, similarly to the culture-dependent method, accounting for 53% of the entire bacterial clone library. Our results indicate that endophytic bacteria diversity in the Tarim River Basin was rich, and the resources of endophytic bacteria were high. They provide valuable reference data and species resources for screening indigenous and functional strains of endophytic bacteria in .
对塔里木河流域沙雅县和于田县的 茎贮藏液样品中的细菌多样性进行了调查。采用了一种基于培养的（稀释平板法）和非培养的方法（PCR-RFLP 技术）来鉴定塔里木河流域 中的内生细菌群落结构和组成。从三种琼脂培养基上分离出 66 株来自 茎贮藏液样品的细菌菌株。使用细菌通用引物扩增和测序 16S rDNA 基因。系统发育分析表明，这 66 株菌属于三个门（Firmicutes、Actinomycetes 和 Gamma-Proteobacteria），包括 16 个属和 29 个种。其中， （27.27%）和 （19.69%）是优势分离株。CGM-17 是一种潜在的新种 。聚合酶链反应（PCR）扩增的 16S rDNA 基因的限制性片段长度多态性（PCR-RFLP）显示了 48 个操作分类单元（OTUs）。系统发育分析表明，这 48 个 OTUs 属于Firmicutes、Actinobacteria、Proteobacteria（α-、β-、γ-亚群）、Bacteroidetes 和 Verrucomicrobia。与基于培养的方法相似，Gamma-Proteobacteria 是优势群体，占整个细菌克隆文库的 53%。我们的研究结果表明，塔里木河流域的 内生细菌多样性丰富，内生细菌资源丰富。它们为筛选塔里木河流域 中的本土和功能内生细菌提供了有价值的参考数据和物种资源。
