褪黑素上调着床前小鼠胚胎中的两种主要着床受体ErbB1和ErbB4。
Melatonin upregulates ErbB1 and ErbB4, two primary implantation receptors, in pre-implantation mouse embryos.
作者信息
Moshkdanian Ghazaleh, Moghani-Ghoroghi Fatemeh, Pasbakhsh Parichehr, Nematollahi-Mahani Seyed Noureddin, Najafi Atefeh, Kashani sIraj Ragerdi
机构信息
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Anatomical Science Research Center, Kashan University of Medical Sciences, Kashan, Iran.
出版信息
Iran J Basic Med Sci. 2017 Jun;20(6):655-661. doi: 10.22038/IJBMS.2017.8833.
OBJECTIVES
To evaluated the effects of melatonin on early embryo competence and the expression rate of the primary implantation receptors (ErbB1 and ErbB4).
MATERIALS AND METHODS
Two-cell mouse embryos were cultured in 3 groups: simple media, melatonin-treated (10 M melatonin) and Luzindole-treated (10 M luzindole). Then, the rate of ErbB1 and ErbB4 gene and protein expression, the level of intracellular ROS, antioxidant capacity, and also the number of cells were evaluated and compared with the fourth group developed blastocysts (control group).
RESULTS
We concluded that melatonin significantly up-regulated the ErbB1 and ErbB4 gene and protein expression, decreased intracellular ROS, increased the total antioxidant capacity, and also elevated the cell numbers in the melatonin-treated group compared with the other groups (≤ 0.05).
CONCLUSION
The use of melatonin may be a helpful factor in improving the embryo quality and enhancing the expression of ErbB1 and ErbB4, two important implantation-related genes and proteins.
目的
评估褪黑素对早期胚胎发育能力以及主要着床受体（ErbB1和ErbB4）表达率的影响。
材料与方法
将二细胞期小鼠胚胎分为3组培养：单纯培养基组、褪黑素处理组（10 μM褪黑素）和鲁辛朵尔处理组（10 μM鲁辛朵尔）。然后，评估并比较ErbB1和ErbB4基因及蛋白表达率、细胞内活性氧水平、抗氧化能力以及细胞数量，并与第四组发育为囊胚的胚胎（对照组）进行比较。
结果
我们得出结论，与其他组相比，褪黑素处理组中褪黑素显著上调了ErbB1和ErbB4基因及蛋白表达，降低了细胞内活性氧水平，提高了总抗氧化能力，还增加了细胞数量（P≤0.05）。
结论
使用褪黑素可能是改善胚胎质量以及增强ErbB1和ErbB4这两个重要的着床相关基因和蛋白表达的一个有益因素。
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