Azizi Faezeh, Saleh Gargari Soraya, Asadi Shahmirzadi Sedigheh, Dodange Fatemeh, Amiri Vahid, Mirfakhraie Reza, Omrani Mir Davood
Department of Medical Genetics, Faculty of Medicine, ShahidBeheshtiUniversity of Medical Sciences,Tehran, Iran.
Feto-Maternal Unit, ShohadayeTajrish Hospital, ShahidBeheshti, University of Medical Sciences, Tehran, Iran.
Int J Mol Cell Med. 2017 Winter;6(1):22-30. Epub 2017 Jan 17.
It has been well documented that preeclampsia (PE) has a common etiological background, but little is known about its linkage at the molecular level.Non- coding RNAs are critical posttranscriptional regulators ofgene expression. This study was performed to determine whether PE is associated with alterations in placental non-coding RNAs expression. MicroRNA (miR)-155-5p and long non-coding RNA () expression, in placentas collected sequentially from 59 patients with PE and 40 normotensive pregnancies were measured using real-time PCR.The relationship between miR-155-5p and expressions was analyzed statistically. miR-155-5p expression was increased (fold change =1.6, P=0.04), while lncsONE expression was not significantly changed (fold change =1.1, P=0.68), in placentas from patients compared with control group.miR-155-5p was upregulated in placentas from patients with PE and may have influenced expression. These findings indicate that miRNA-155-5p may be involved in PE pathogenesis and could be a potential biomarker for this disease.
已有充分文献记载，子痫前期（PE）具有共同的病因背景，但在分子水平上其关联尚不清楚。非编码RNA是基因表达的关键转录后调节因子。本研究旨在确定PE是否与胎盘非编码RNA表达的改变有关。使用实时PCR检测了从59例PE患者和40例血压正常的孕妇中依次收集的胎盘组织中微小RNA（miR）-155-5p和长链非编码RNA（lncRNA）的表达。对miR-155-5p和lncRNA的表达关系进行了统计学分析。与对照组相比，患者胎盘组织中miR-155-5p表达增加（倍数变化=1.6，P=0.04），而lncRNA lncSONE表达无明显变化（倍数变化=1.1，P=0.68）。PE患者胎盘中miR-155-5p上调，可能影响了lncSONE的表达。这些发现表明，miRNA-155-5p可能参与PE的发病机制，可能是该疾病的潜在生物标志物。
