Department of Clinical Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Medical Genetics Laboratory, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Biochem Genet. 2023 Oct;61(5):2149-2158. doi: 10.1007/s10528-023-10364-2. Epub 2023 Mar 26.
Hypertension (HTN) is a global health challenge and increase the risk of cardiovascular disease. Hypertension has a multifactorial course of evolution, with both genetic and environmental factors playing an important role. To date, a number of genes and pathways have been proposed to be associated with HTN, among which is Nitric Oxide pathway. NO levels can be regulated by reactive oxygen species (ROS), superoxide and post-transcriptional mechanisms, including sense-anti sense interactions. NOS3AS gene encodes an antisense RNA (sONE) which is complementary to NOS3 transcript in 662 nucleotides and may regulate NOS3 in a post-transcriptional manner. In this study, we sought to define the role of NOS3AS in the pathophysiology of essential HTN. A total of 131 cases with hypertension and 115 controls were enrolled in the study. Peripheral blood was drawn from all study participants after signing the informed consent form. Three variants (rs71539868, rs12666075 and rs7830) were investigated by Tetra-ARMS PCR method. The results were then statistically analyzed. We found statistically significant association between rs7830 TT genotype, rs12666075 GT and TT genotypes with susceptibility to HTN. We failed to observe association between rs71539868 and susceptibility to HTN. The present study showed a strong association between NOS3AS variants and susceptibility to hypertension in the population of Kermanshah province. Our results may shed more light on the mechanisms of disease development and may also help to better identify genetic predispositions and individuals at risk.
高血压（HTN）是一个全球性的健康挑战，增加了心血管疾病的风险。高血压的演变具有多因素的特点，遗传和环境因素都起着重要作用。迄今为止，已经提出了许多与 HTN 相关的基因和途径，其中包括一氧化氮途径。NO 水平可以通过活性氧（ROS）、超氧化物和转录后机制（包括反义-反义相互作用）进行调节。NOS3AS 基因编码一种反义 RNA（sONE），与 NOS3 转录物互补 662 个核苷酸，可能以转录后方式调节 NOS3。在这项研究中，我们试图确定 NOS3AS 在原发性高血压病理生理学中的作用。共纳入 131 例高血压患者和 115 例对照者。所有研究参与者在签署知情同意书后采集外周血。采用 Tetra-ARMS PCR 法检测 3 个变异体（rs71539868、rs12666075 和 rs7830）。然后对结果进行统计学分析。我们发现 rs7830 TT 基因型、rs12666075 GT 和 TT 基因型与高血压易感性之间存在统计学显著关联。我们没有观察到 rs71539868 与高血压易感性之间的关联。本研究显示 NOS3AS 变异与克尔曼沙阿省人群高血压易感性之间存在很强的关联。我们的研究结果可能为疾病发展的机制提供更多的线索，并有助于更好地识别遗传易感性和高危个体。
