肿瘤性种系突变体生殖细胞的原代培养系统
Primary Culture System for Germ Cells from Tumorous Germline Mutants.
作者信息
Vagasi Alexandra S, Rahman Mohammad M, Chaudhari Snehal N, Kipreos Edward T
机构信息
Department of Cellular Biology, University of Georgia, Athens, GA, USA.
Department of Genetics, University of Georgia, Athens, GA, USA.
出版信息
Bio Protoc. 2017 Aug 5;7(15). doi: 10.21769/BioProtoc.2424.
The germ line is an important model system for the study of germ stem cells. Wild-type germ cells are syncytial and therefore cannot be isolated in cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact from the mutant animals. Here we describe a detailed protocol for the isolation of germ cells from tumorous mutants that allows the germ cells to be maintained for extended periods in an primary culture. This protocol has been adapted from Chaudhari ., 2016.
生殖系是研究生殖干细胞的重要模型系统。野生型生殖细胞是多核的，因此不能在培养物中分离出来。相比之下，来自肿瘤突变体的生殖细胞可以完全细胞化，并从突变动物中完整分离出来。在这里，我们描述了一种从肿瘤突变体中分离生殖细胞的详细方案，该方案允许生殖细胞在原代培养中长时间维持。该方案改编自Chaudhari等人，2016年。
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