肿瘤启动剂（蜂毒素、远藤霉素和岩沙海葵毒素）对单层培养的大鼠垂体前叶细胞生长激素分泌的影响。

Effects of tumor promoters (mezerein, teleocidin and palytoxin) on growth hormone secretion from rat anterior pituitary cells cultured in monolayer.

作者信息

Ohmura E, Tsushima T, Emoto N, Obuchi E, Shizume K

出版信息

Life Sci. 1987 Aug 10;41(6):691-6. doi: 10.1016/0024-3205(87)90448-6.

Abstract

The effects of compounds with tumor promoting activity (mezerein, teleocidin and palytoxin) on rat growth hormone (rGH) release was compared to that of TPA (12-O-tetradecanoyl phorbol-13-acetate). Mezerein and teleocidin both of which are activators of protein kinase C (TPA type tumor promoter), elicited rGH release about 3.5 to 4 fold above control values. The ED 50 was 16 nM for mezerein, 1.1 nM for teleocidin and 1.5 nM for TPA. In contrast to mezerein or teleocidin, a non-TPA type tumor promoter (palytoxin) which does not activate protein kinase C failed to stimulate rGH release. These observations suggest that the activation of protein kinase C is essential in the release of rGH induced by the tumor promoters.

摘要

将具有促肿瘤活性的化合物（芫花酯甲、远藤菌素和岩沙海葵毒素）对大鼠生长激素（rGH）释放的影响与12-O-十四酰佛波醇-13-乙酸酯（TPA）进行了比较。芫花酯甲和远藤菌素均为蛋白激酶C的激活剂（TPA类肿瘤促进剂），它们引起的rGH释放比对照值高约3.5至4倍。芫花酯甲的半数有效剂量（ED50）为16 nM，远藤菌素为1.1 nM，TPA为1.5 nM。与芫花酯甲或远藤菌素不同，一种不激活蛋白激酶C的非TPA类肿瘤促进剂（岩沙海葵毒素）未能刺激rGH释放。这些观察结果表明，蛋白激酶C的激活在肿瘤促进剂诱导的rGH释放中至关重要。