肿瘤启动剂诱导小鼠腹腔巨噬细胞还原硝基蓝四氮唑以及某些抑制剂对诱导的硝基蓝四氮唑还原的抑制作用。

Induction of nitroblue tetrazolium reduction in mouse peritoneal macrophages by tumour promoters and inhibition of the induced nitroblue tetrazolium reduction by some inhibitors.

作者信息

Ohkawa Y, Iwata K, Shibuya H, Inui N

出版信息

Cancer Lett. 1985 Jul;27(3):261-7. doi: 10.1016/0304-3835(85)90183-1.

Abstract

Two polyacetates, aplysiatoxin and debromoaplysiatoxin, as well as 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein and teleocidin enhance nitroblue tetrazolium (NBT) reduction in mouse peritoneal macrophages in vitro. The ED50 values for NBT reduction of these 5 TPA-type tumor promoters were 4.2 ng/ml for TPA, 36 ng/ml for mezerein, 0.53 ng/ml for teleocidin, 1.5 ng/ml for aplysiatoxin and 108 ng/ml for debromoaplysiatoxin. The NBT reduction induced by the 5 tumor promoters is inhibited by 2 inhibitors of tumor promotion, retinoic acid and dibromoacetophenone. The possibility that tumor promotion by TPA-type tumor promoters involves similar mechanisms such as superoxide anion radicals release in cell membranes is discussed.

摘要

两种聚乙酸酯，即海兔毒素和脱溴海兔毒素，以及12-O-十四烷酰佛波醇-13-乙酸酯（TPA）、瑞香毒素和远侧霉素在体外均可增强小鼠腹腔巨噬细胞对硝基蓝四唑（NBT）的还原作用。这5种TPA型肿瘤促进剂对NBT还原的半数有效剂量（ED50）分别为：TPA为4.2 ng/ml，瑞香毒素为36 ng/ml，远侧霉素为0.53 ng/ml，海兔毒素为1.5 ng/ml，脱溴海兔毒素为108 ng/ml。5种肿瘤促进剂所诱导的NBT还原作用受到两种肿瘤促进作用抑制剂（视黄酸和二溴苯乙酮）的抑制。文中讨论了TPA型肿瘤促进剂的肿瘤促进作用涉及细胞膜中释放超氧阴离子自由基等类似机制的可能性。