Sandberg M, Hagberg H, Jacobson I, Karlsson B, Lehmann A, Hamberger A
Life Sci. 1987 Aug 17;41(7):829-32. doi: 10.1016/0024-3205(87)90173-1.
For high sensitivity analysis of neuroactive amino acids, liquid chromatography employing precolumn derivatisation with o-phthalaldehyde (OPA) is suitable for several reasons. The OPA reagent is non-fluorescent per se, the reaction occurs rapidly in alkaline aqueous solutions and forms highly fluorescent derivatives with primary amines.
对于神经活性氨基酸的高灵敏度分析，采用邻苯二甲醛（OPA）进行柱前衍生化的液相色谱法是合适的，原因如下。OPA试剂本身无荧光，该反应在碱性水溶液中迅速发生，并与伯胺形成高荧光衍生物。
