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通过稳定的邻苯二甲醛-亚硫酸盐衍生物的高效液相色谱法对人脑中神经递质氨基酸进行电化学检测。

Electrochemical detection of human brain transmitter amino acids by high-performance liquid chromatography of stable o-phthalaldehyde-sulphite derivatives.

作者信息

Pearson S J, Czudek C, Mercer K, Reynolds G P

机构信息

Department of Biomedical Science, University of Sheffield, United Kingdom.

出版信息

J Neural Transm Gen Sect. 1991;86(2):151-7. doi: 10.1007/BF01250576.

DOI:10.1007/BF01250576
PMID:1683240
Abstract

A simple, sensitive, reliable and reproducible isocratic HPLC technique for the measurement of OPA/sulphite derivatives of human brain amino acid neurotransmitters is described. This employs a sample preparation that is also compatible with the concurrent determination of monoamines and their metabolites on a separate HPLC system. The method has been applied to the determination of GABA and glutamate in brain tissue taken post-mortem from patients with Huntington's disease and control subjects.

摘要

本文描述了一种用于测定人脑海氨基酸神经递质的邻苯二甲醛/亚硫酸盐衍生物的简单、灵敏、可靠且可重复的等度高效液相色谱技术。该技术采用的样品制备方法也适用于在单独的高效液相色谱系统上同时测定单胺及其代谢物。该方法已应用于测定亨廷顿舞蹈病患者和对照受试者死后脑组织中的γ-氨基丁酸和谷氨酸。

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1
Electrochemical detection of human brain transmitter amino acids by high-performance liquid chromatography of stable o-phthalaldehyde-sulphite derivatives.通过稳定的邻苯二甲醛-亚硫酸盐衍生物的高效液相色谱法对人脑中神经递质氨基酸进行电化学检测。
J Neural Transm Gen Sect. 1991;86(2):151-7. doi: 10.1007/BF01250576.
2
Determination of in vivo amino acid neurotransmitters by high-performance liquid chromatography with o-phthalaldehyde-sulphite derivatisation.采用邻苯二甲醛-亚硫酸盐衍生化高效液相色谱法测定体内氨基酸神经递质。
J Neurosci Methods. 1995 Mar;57(1):93-9. doi: 10.1016/0165-0270(94)00132-z.
3
An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.一种改进的快速高效液相色谱-电化学检测法，用于从脑组织和微透析灌注液中进行氨基酸神经递质的等度分离。
Life Sci. 1988;43(11):913-22. doi: 10.1016/0024-3205(88)90267-6.
4
Measurement of GABA in rat brain microdialysates using o-phthaldialdehyde-sulphite derivatization and high-performance liquid chromatography with electrochemical detection.使用邻苯二甲醛-亚硫酸盐衍生化和高效液相色谱-电化学检测法测定大鼠脑微透析液中的γ-氨基丁酸
J Chromatogr. 1994 Feb 11;652(2):228-33. doi: 10.1016/0378-4347(93)e0391-3.
5
Rapid method for micro-analysis of endogenous amino acid neurotransmitters in brain perfusates in the rat by isocratic HPLC-EC.大鼠脑灌注液中内源性氨基酸神经递质等度高效液相色谱-电化学检测微分析的快速方法。
J Neurosci Methods. 1986 Nov;18(3):269-76. doi: 10.1016/0165-0270(86)90013-0.
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Simultaneous determination of amino acids in discrete brain areas in Suncus murinus by high performance liquid chromatography with electrochemical detection.高效液相色谱电化学检测法同时测定沙鼠脑区氨基酸。
J Pharm Biomed Anal. 2010 Nov 2;53(3):705-9. doi: 10.1016/j.jpba.2010.04.001. Epub 2010 Apr 9.
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Electrochemical activity of o-phthalaldehyde-mercaptoethanol derivatives of amino acids. Application to high-performance liquid chromatographic determination of amino acids in plasma and other biological materials.氨基酸的邻苯二甲醛-巯基乙醇衍生物的电化学活性。应用于高效液相色谱法测定血浆及其他生物材料中的氨基酸。
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Determination of amino acids in the brain by high-performance liquid chromatography with isocratic elution and electrochemical detection.采用等度洗脱高效液相色谱法和电化学检测法测定脑中的氨基酸。
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Analysis of amino acids: neurochemical application.氨基酸分析：神经化学应用。
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Simultaneous determination of total and extracellular concentrations of the amino acid neurotransmitters in cat visual cortex by microbore liquid chromatography and electrochemical detection.采用微径液相色谱和电化学检测法同时测定猫视觉皮层中氨基酸神经递质的总浓度和细胞外浓度。
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本文引用的文献

1
A FURTHER STUDY OF THE DISTRIBUTION OF GAMMA-AMINOBUTYRIC ACID BETWEEN EXCITATORY AND INHIBITORY AXONS OF THE LOBSTER.龙虾兴奋性和抑制性轴突之间γ-氨基丁酸分布的进一步研究
J Neurochem. 1965 Apr;12:323-8. doi: 10.1111/j.1471-4159.1965.tb06768.x.
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Determination of content and specific activity of amino acids in central nervous system tissue utilizing tritium and carbon-14 dual labeling.利用氚和碳-14双标记法测定中枢神经系统组织中氨基酸的含量和比活性。
Anal Biochem. 1980 Jul 15;106(1):191-4. doi: 10.1016/0003-2697(80)90136-0.
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Determination of gamma-aminobutyric acid by liquid chromatography with electrochemical detection.
急性缺血性损伤患者脑脊液中神经递质氨基酸水平
Neurosci Behav Physiol. 2000 Sep-Oct;30(5):491-5. doi: 10.1007/BF02462604.
4
Neocortical neurotransmitter markers in Huntington's disease.亨廷顿舞蹈病中的新皮质神经递质标志物
J Neural Transm Gen Sect. 1994;98(3):197-207. doi: 10.1007/BF01276536.
采用液相色谱-电化学检测法测定γ-氨基丁酸。
J Chromatogr. 1982 Feb 12;227(2):331-9. doi: 10.1016/s0378-4347(00)80387-4.
4
o-Phthalaldehyde derivatives of amines for high-speed liquid chromatography/electrochemistry.用于高效液相色谱/电化学的胺类邻苯二甲醛衍生物
Anal Chem. 1984 Jun;56(7):1089-96. doi: 10.1021/ac00271a010.
5
Electrochemical activity of o-phthalaldehyde-mercaptoethanol derivatives of amino acids. Application to high-performance liquid chromatographic determination of amino acids in plasma and other biological materials.氨基酸的邻苯二甲醛-巯基乙醇衍生物的电化学活性。应用于高效液相色谱法测定血浆及其他生物材料中的氨基酸。
J Chromatogr. 1983 Oct 14;277:125-36.
6
Rapid analysis of amino acids using pre-column derivatization.采用柱前衍生化法快速分析氨基酸。
J Chromatogr. 1984 Dec 7;336(1):93-104. doi: 10.1016/s0378-4347(00)85133-6.
7
Regional distribution of amino acids in human brain obtained at autopsy.尸检获得的人类大脑中氨基酸的区域分布。
J Neurochem. 1971 Mar;18(3):513-9. doi: 10.1111/j.1471-4159.1971.tb11979.x.
8
Amino acid analysis with fluorescamine at the picomole level.皮摩尔水平的荧光胺氨基酸分析。
Arch Biochem Biophys. 1973 Mar;155(1):202-12. doi: 10.1016/s0003-9861(73)80022-0.
9
Huntington's chorea. Deficiency of gamma-aminobutyric acid in brain.亨廷顿舞蹈病。大脑中γ-氨基丁酸缺乏。
N Engl J Med. 1973 Feb 15;288(7):337-42. doi: 10.1056/NEJM197302152880703.
10
o-Phthalaldehyde-sulfite derivatization of primary amines for liquid chromatography-electrochemistry.用于液相色谱 - 电化学的伯胺的邻苯二甲醛 - 亚硫酸盐衍生化
J Chromatogr. 1987 Apr 17;392:435-41. doi: 10.1016/s0021-9673(01)94288-8.