Ungerstedt U, Hallström A
Life Sci. 1987 Aug 17;41(7):861-4. doi: 10.1016/0024-3205(87)90181-0.
Microdialysis is a new technique to monitor levels of chemical compounds in the extracellular space over time. It involves the implantation of a microdialysis probe into the brain tissue. The probe is similar to a push-pull probe but the perfusate is contained inside a semi-permeable membrane located at the tip of the probe. Substances in the extracellular fluid will diffuse into the perfusate while substances included in the perfusate will diffuse into the tissue. This principle opens up a wealth of possibilities to monitor chemical events within the brain and to study the working mechanism of various drugs. The perfusate may be analysed by a number of different techniques. In this paper we give a short summary of various HPLC techniques that have proven particularly useful.
微透析是一种用于长期监测细胞外空间中化合物水平的新技术。它涉及将微透析探针植入脑组织。该探针类似于推挽式探针，但灌注液包含在位于探针尖端的半透膜内。细胞外液中的物质会扩散到灌注液中，而灌注液中的物质会扩散到组织中。这一原理为监测脑内化学事件和研究各种药物的作用机制开辟了众多可能性。灌注液可以通过多种不同技术进行分析。在本文中，我们简要总结了已证明特别有用的各种高效液相色谱技术。
