Ramgir Shalaka S, Sekar Nishu, Jindam Divya, V G Abilash
Department of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu-632014, India.
Department of Biomedical Sciences, School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamilnadu-632014, India.
Int J Fertil Steril. 2017 Oct;11(3):142-147. doi: 10.22074/ijfs.2017.4752. Epub 2017 Aug 27.
Infertility is the inability of a couple to conceive after one and a half years of unprotected sex. Male infertility, which accounts for almost half of infertility cases, is considered as a major problem all over the world. The aim of this study was to investigate the association of CYP1A1 polymorphisms with idiopathic non-obstructive azoospermia in a South Indian cohort.
An experimental study was conducted with idiopathic nonobstructive azoospermia. A total of 120 infertile and 80 fertile samples were collected, and DNA was then extracted from all samples. The CYP1A1*2A polymorphism genotyping was carried out by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).
The genotype distribution of CYP1A1*2A polymorphism showed significant difference between patients and controls. Moreover, the CC genotype was associated with decreased risk of idiopathic non-obstructive azoospermia in comparison with the TT and TC genotypes.
The current experimental study identified that the CT genotype of CYP1A1*2A polymorphism may contribute to the pathogenesis of male infertility in the South Indian population.
不孕症是指一对夫妇在无保护性行为一年半后仍无法受孕。男性不育症占不孕症病例的近一半，被认为是全球的一个主要问题。本研究的目的是调查南印度人群中CYP1A1基因多态性与特发性非梗阻性无精子症的关联。
对特发性非梗阻性无精子症进行了一项实验研究。共收集了120份不育样本和80份可育样本，然后从所有样本中提取DNA。通过聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）对CYP1A1*2A基因多态性进行基因分型。
CYP1A1*2A基因多态性的基因型分布在患者和对照组之间存在显著差异。此外，与TT和TC基因型相比，CC基因型与特发性非梗阻性无精子症风险降低相关。
目前的实验研究表明，CYP1A1*2A基因多态性的CT基因型可能在南印度人群男性不育症的发病机制中起作用。
