Zhu X Z, Chuang D M
Life Sci. 1987 Aug 31;41(9):1133-9. doi: 10.1016/0024-3205(87)90632-1.
Our previous study demonstrated that long term treatment of NCB-20 cells with sodium butyrate resulted in a marked increase in the density of delta-opioid receptors with a much lesser effect on muscarinic cholinergic and no effect on alpha 2-adrenergic receptors. In the present study we investigated the effect of sodium butyrate on these three types of receptors in NG108-15 cells whose neuroblastoma parent is the same as that of NCB-20 cells. Long term treatment of NG108-15 cells with sodium butyrate (0.5 mM) induced a 2-fold increase in the density of the specific binding of 3H-clonidine. A comparable increase in the number of binding sites was detected when 3H-yohimbine was used as the receptor ligand. The butyrate-induced increase in the alpha 2-adrenergic receptor binding could be totally abolished by treatment with a protein synthesis inhibitor, cycloheximide, suggesting that synthesis of receptor protein is involved. The same butyrate treatment had no significant effect on opioid and muscarinic cholinergic receptor bindings. Thus, butyrate effects on the expression of these three types of receptors in NG108-15 and NCB-20 cells are dramatically different. These data suggest that induction by butyrate of neurotransmitter receptors requires concerted action of genetic factors of both parents of the neurohybrids.
我们之前的研究表明，用丁酸钠对NCB - 20细胞进行长期处理会导致δ - 阿片受体密度显著增加，对毒蕈碱胆碱能受体的影响小得多，而对α2 - 肾上腺素能受体则无影响。在本研究中，我们研究了丁酸钠对NG108 - 15细胞中这三种类型受体的影响，该细胞的神经母细胞瘤亲本与NCB - 20细胞相同。用丁酸钠（0.5 mM）对NG108 - 15细胞进行长期处理会使3H - 可乐定特异性结合的密度增加2倍。当使用3H - 育亨宾作为受体配体时，检测到结合位点数量有类似增加。丁酸盐诱导的α2 - 肾上腺素能受体结合增加可以通过用蛋白质合成抑制剂环己酰亚胺处理而完全消除，这表明受体蛋白的合成参与其中。相同的丁酸盐处理对阿片样物质和毒蕈碱胆碱能受体结合没有显著影响。因此，丁酸盐对NG108 - 15和NCB - 20细胞中这三种类型受体表达的影响显著不同。这些数据表明，丁酸盐对神经杂交体双亲遗传因素的协同作用是神经递质受体诱导所必需的。
