聚己内酯/壳聚糖纳米粒为乙肝抗原提供了强大的佐剂效应。
Poly-ϵ-caprolactone/chitosan nanoparticles provide strong adjuvant effect for hepatitis B antigen.
机构信息
Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
Center for Neuroscience & Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
出版信息
Nanomedicine (Lond). 2017 Oct;12(19):2335-2348. doi: 10.2217/nnm-2017-0138. Epub 2017 Sep 4.
AIM
This work aims to investigate the adjuvant effect of poly-ϵ-caprolactone/chitosan nanoparticles (NPs) for hepatitis B surface antigen (HBsAg) and the plasmid DNA encoding HBsAg (pRC/CMV-HBs).
METHODS
Both antigens were adsorbed onto preformed NPs. Vaccination studies were performed in C57BL/6 mice. Transfection efficiency was investigated in A549 cell line.
RESULTS
HBsAg-adsorbed NPs generated strong anti-HBsAg IgG titers, mainly of IgG1 isotype, and induced antigen-specific IFN-γ and IL-17 secretion by spleen cells. The addition of pRC/CMV-HBs to the HBsAg-adsorbed NPs inhibited IL-17 secretion but had minor effect on IFN-γ levels. Lastly, pRC/CMV-HBs-loaded NPs generated a weak serum antibody response.
CONCLUSION
Poly-ϵ-caprolactone/chitosan NPs provide a strong humoral adjuvant effect for HBsAg and induce a Th1/Th17-mediated cellular immune responses worth explore for hepatitis B virus vaccination.
目的
本研究旨在探讨聚己内酯/壳聚糖纳米粒（NPs）对乙型肝炎表面抗原（HBsAg）和乙型肝炎表面抗原编码质粒 DNA（pRC/CMV-HBs）的佐剂效应。
方法
将两种抗原吸附到预先形成的 NPs 上。在 C57BL/6 小鼠中进行疫苗接种研究。在 A549 细胞系中研究转染效率。
结果
HBsAg 吸附的 NPs 产生了强烈的抗 HBsAg IgG 滴度，主要为 IgG1 同种型，并诱导脾细胞产生抗原特异性 IFN-γ 和 IL-17 分泌。将 pRC/CMV-HBs 添加到 HBsAg 吸附的 NPs 中抑制了 IL-17 的分泌，但对 IFN-γ 水平的影响较小。最后，负载 pRC/CMV-HBs 的 NPs 产生了较弱的血清抗体反应。
结论
聚己内酯/壳聚糖 NPs 为 HBsAg 提供了强烈的体液佐剂效应，并诱导了 Th1/Th17 介导的细胞免疫反应，值得进一步探索用于乙型肝炎病毒疫苗接种。
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