Scudiero Rosaria, Verderame Mariailaria
Department of Biology, University of Naples Federico II, via Mezzocannone 8, 80134 Napoli, Italy.
Department of Biology, University of Naples Federico II, via Mezzocannone 8, 80134 Napoli, Italy.
C R Biol. 2017 Aug;340(8):372-378. doi: 10.1016/j.crvi.2017.08.001. Epub 2017 Aug 30.
The "sex-hormone" estrogen-17β promotes several cognitive functions and is a master regulator of brain bioenergetics via the estrogen receptors α and β (ERα and ERβ). In this work, by using Real-Time PCR analysis, we evaluated the effect of aging and high fat diet (HFD) on ERα and ERβ expression in rat hippocampus and cortex. In young rats, ERβ is abundant in cortex and ERα in hippocampus. During the aging, in the cortex, we observe a general decrease in ERα and ERβ expression; in hippocampus ERα increases and ERβ decreases. ER expression patterns in rat brain are also affected by the administration of an HFD. In cortex, after 4weeks of HFD, ERβ transcripts are down-regulated, whereas ERα levels remain unchanged; after 12weeks, both ERα and ERβ expression is up-regulated. In the hippocampus, the level of ERβ transcripts does not change following HDF, whereas ERα expression is affected by HDF, in a time-dependent manner: it increases after the 4-week treatment and decreases after 12weeks. Possible involvements of these receptors in the control of cortex and hippocampus functions during aging and in the modulation of energetic metabolism and feeding behaviour are discussed.
“性激素”17β-雌二醇可促进多种认知功能，并且通过雌激素受体α和β（ERα和ERβ）成为脑生物能量学的主要调节因子。在本研究中，我们通过实时定量PCR分析，评估了衰老和高脂饮食（HFD）对大鼠海马体和皮质中ERα和ERβ表达的影响。在年轻大鼠中，皮质中ERβ丰富，海马体中ERα丰富。在衰老过程中，在皮质中，我们观察到ERα和ERβ表达普遍下降；在海马体中，ERα增加而ERβ减少。大鼠脑中的ER表达模式也受到高脂饮食的影响。在皮质中，高脂饮食4周后，ERβ转录本下调，而ERα水平保持不变；12周后，ERα和ERβ表达均上调。在海马体中，高脂饮食后ERβ转录本水平不变，而ERα表达受高脂饮食影响，呈时间依赖性：4周治疗后增加，12周后减少。本文讨论了这些受体在衰老过程中对皮质和海马体功能的控制以及对能量代谢和摄食行为的调节中可能发挥的作用。
