Rong Jin-Cheng, Cha Qian-Qian, Ren Xue-Bing, Xie Bin-Bin, Song Xiao-Yan
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China.
State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China; Institute of Marine Science and Technology, Shandong University, Jinan 250100, China.
Mar Genomics. 2018 Apr;38:21-23. doi: 10.1016/j.margen.2017.08.006. Epub 2017 Aug 30.
Strain DSM9414, the type strain of Pseudoalteromonas espejiana, is a Gram-negative, and amino-acid-requiring stain isolated from seawater off the coast of Northern California. In this study, we report the complete genome sequence of Pseudoalteromonas espejiana DSM9414. The genome (4,500,451bp; 40.3% G+C) is composed of two circular chromosomes: chromosome I is 3,720,756bp with 40.4% G+C content and chromosome II is 779,695bp with 39.8% G+C content. Genomic analysis showed that chromosome I encodes a complete set of ABC transporters responsible for branched-chain amino acids, whose homologous proteins were not discovered in other Pseudoalteromonas genomes released. This result indicated the tight dependence of extracellular amino acids for strain DSM9414, which is consistent with its phenotype. The complete genome sequence of P. espejiana provides further genetic insights into the diversity of dependence on extracellular amino acids for Pseudoalteromonas species.
菌株DSM9414是埃氏假交替单胞菌（Pseudoalteromonas espejiana）的模式菌株，为革兰氏阴性菌，是一株从北加利福尼亚海岸外海水中分离得到的需要氨基酸的菌株。在本研究中，我们报道了埃氏假交替单胞菌DSM9414的全基因组序列。该基因组（4,500,451bp；G+C含量为40.3%）由两条环状染色体组成：染色体I为3,720,756bp，G+C含量为40.4%，染色体II为779,695bp，G+C含量为39.8%。基因组分析表明，染色体I编码了一套完整的负责支链氨基酸的ABC转运蛋白，在已公布的其他假交替单胞菌基因组中未发现其同源蛋白。这一结果表明菌株DSM9414对细胞外氨基酸的紧密依赖性，这与其表型一致。埃氏假交替单胞菌的全基因组序列为深入了解假交替单胞菌属对细胞外氨基酸依赖性的多样性提供了进一步的遗传学见解。
