Complete genome analysis of copper resistant bacteria Pseudoalteromonas sp. CuT4-3 isolated from a deep-sea hydrothermal vent.

Pseudoalteromonas sp. CuT4-3, a copper resistant bacterium, was isolated from deep-sea hydrothermal sulfides on the Southwest Indian Ridge (SWIR), is an aerobic, mesophilic and rod-shaped bacterium belonging to the family Pseudoalteromonadaceae (class Gammaproteobacteria, order Alteromonadales). In this study, we present the complete genome sequence of strain CuT4-3, which consists of a single circular chromosome comprising 3,660,538 nucleotides with 41.05% G + C content and two circular plasmids comprising 792,064 nucleotides with 40.36% G + C content and 65,436 nucleotides with 41.50% G + C content. In total, 4078 protein coding genes, 105 tRNA genes, and 25 rRNA genes were obtained. Genomic analysis of strain CuT4-3 identified numerous genes related to heavy metal resistance (especially copper) and EPS production. The genome of strain CuT4-3 will be helpful for further understanding of its adaptive strategies, particularly its ability to resist heavy metal, in the deep-sea hydrothermal vent environment.