School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Singapore Eye Research Institute, 11 Third Hospital Ave, Singapore, 168751, Singapore.
J Biomed Mater Res A. 2017 Dec;105(12):3502-3513. doi: 10.1002/jbm.a.36198. Epub 2017 Oct 11.
The manufacture of mechanically strong and biocompatible titania (TiO ) materials is of vital importance for their application as corneal implant skirts. This study was aimed at optimizing the selection of raw powder and sintering conditions for TiO ceramics. TiO compacts were synthesized from five raw powders, denoted as Altair, Inframat, Alfa, Materion, and Amperit, respectively, by spark plasma sintering using different sintering parameters. The XRD and Raman results confirmed that the anatase TiO phase in the Inframat powder had converted completely to rutile TiO phase after sintering at 900°C and above. The nanoindentation results indicated that among the five types of TiO samples sintered at 1100°C, the Inframat pellets possessed the highest Young's modulus and hardness. Additionally, when Materion samples were employed to study the effects of SPS parameters, a higher sintering temperature in the range of 1100-1300°C decreased the mechanical properties of sintered pellets probably due to the generation of more structural defects. Culture of human corneal stromal fibroblasts on the sintered sample surfaces showed that comparably high cell viability and proliferation were observed on all TiO samples except Amperit compared to positive control. Furthermore, cells cultured on Inframat TiO sintered in the temperature range of 900-1300°C exhibited viability and formation of focal adhesion complex similar to those on control, and those prepared at 1100°C had significantly higher cell proliferation indices than control. In conclusion, Inframat TiO consolidated at 1100°C by SPS was the best formulation for the preparation of mechanically strong and biocompatible Keratoprosthesis skirt. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3502-3513, 2017.
机械强度高且生物相容性好的二氧化钛（TiO ）材料的制造对于其作为角膜植入裙的应用至关重要。本研究旨在优化 TiO 陶瓷的原料粉末选择和烧结条件。通过使用不同的烧结参数，使用分别称为 Altair、Inframat、Alfa、Materion 和 Amperit 的五种原料粉末，通过火花等离子体烧结合成了 TiO 压坯。XRD 和 Raman 结果证实，Inframat 粉末中的锐钛矿 TiO 相在 900°C 及以上的温度下烧结后已完全转化为金红石 TiO 相。纳米压痕结果表明，在 1100°C 烧结的五种类型的 TiO 样品中，Inframat 压坯具有最高的杨氏模量和硬度。此外，当研究 SPS 参数的影响时，Materion 样品在 1100-1300°C 的较高烧结温度下降低了烧结样品的机械性能，这可能是由于产生了更多的结构缺陷。将人角膜基质成纤维细胞培养在烧结样品表面上，结果表明，与阳性对照相比，除了 Amperit 之外，所有 TiO 样品上均观察到细胞活力和增殖较高。此外，在 900-1300°C 的温度范围内烧结的 Inframat TiO 上培养的细胞表现出与对照相似的活力和焦点粘附复合物的形成，而在 1100°C 下制备的细胞具有比对照更高的细胞增殖指数。总之，通过 SPS 在 1100°C 固结的 Inframat TiO 是制备机械强度高且生物相容性好的角膜假体裙的最佳配方。©2017 Wiley Periodicals，Inc. J Biomed Mater Res Part A：105A：3502-3513，2017。
