Zafari Mandana, Feizabadi Mohamad Mehdi, Jafari Sirous, Abdollahi Alireza, Sabokbar Azar
1 Department of Microbiology, Karaj Branch, Islamic Azad University , Karaj, Iran.
2 Department of Microbiology, School of Medicine, Tehran University of Medical Sciences , Tehran, Iran.
Acta Microbiol Immunol Hung. 2017 Dec 1;64(4):385-394. doi: 10.1556/030.64.2017.031. Epub 2017 Sep 5.
Nosocomial infection caused by carbapenem-resistant Acinetobacter baumannii (CRAB) has created a public health concern all around the world. In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect bla and bla, bla, and bla that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of them were pandrug-resistant (PDR), whereas colistin, polymyxin B, and tigecycline were the most effective agents. All isolates were positive for bla by PCR. The frequency of bla and bla was 81% and 22%, respectively. Findings of this study showed that very few therapeutic options remained for the treatment of CRAB infections and bla is a dominant resistance gene in CRAB at this hospital.
耐碳青霉烯类鲍曼不动杆菌（CRAB）引起的医院感染已在全球范围内引发了公共卫生关注。在本研究中，对2015 - 2016年期间在伊玛目霍梅尼医院住院患者中分离出的100株CRAB进行了调查，采用 Kirby - Bauer 纸片扩散法确定多重耐药（MDR）和广泛耐药（XDR）菌株的比例。通过肉汤微量稀释法测定六种抗生素的最低抑菌浓度（MIC）。进行多重聚合酶链反应（PCR）以检测编码对碳青霉烯类耐药的bla 、bla 、bla 和bla 。所有CRAB分离株均为MDR和XDR，其中2%为全耐药（PDR），而黏菌素、多黏菌素B和替加环素是最有效的药物。通过PCR检测，所有分离株的bla 均呈阳性。bla 和bla 的频率分别为81%和22%。本研究结果表明，治疗CRAB感染的治疗选择非常有限，且bla 是该医院CRAB中的主要耐药基因。
