Molecular Detection of Carbapenemase Genes and Antimicrobial Resistance Patterns among Clinical Isolates of .

is a bacterium found in most places, especially in clinics and hospitals, and an important agent of nosocomial infections. The presence of class D enzymes such as OXA-type carbapenemases in is proven to have a key function in resistance to carbapenem. The aim of the current study is to determine the carbapenemase genes and antimicrobial resistance among clinically isolated samples of We assessed 100 clinically isolated specimens of from patients in intensive care units of educational hospitals of Hamadan, West of Iran. The isolates' susceptibility to antibiotics was performed employing disk diffusion method. Multiplex polymerase chain reaction was used to identify the , , , and genes. , and genes' prevalence were found to be 84, 58, and 3%, respectively. The highest coexistence of the genes was for (84%) followed by (58%). The pattern of genes is a sort of dominant gene in resistance in from Hamadan hospitals. The highest resistance to piperacillin (83%) and ciprofloxacin (81%) has been observed in positive isolates of . The isolates with genes did not show much resistance to antibiotics. Based on the results of the phylogenetic tree analysis, all isolates have shown a high degree of similarity. This study showed the high frequency of -type carbapenemase genes among isolates from Hamadan hospitals, Iran. Thus, applying an appropriate strategy to limit the spreading of these strains and also performing new treatment regimens are necessary.