a Department of Biochemistry , KPC Medical College , Kolkata , India.
b Bio-Organic Division , Bhabha Atomic Research Centre , Mumbai , India.
Free Radic Res. 2017 Jul-Aug;51(7-8):723-738. doi: 10.1080/10715762.2017.1368506. Epub 2017 Sep 5.
In this study, we demonstrated that the cytotoxicity of the protoberberine alkaloids such as coralyne, berberine and jatrorrhizine to several human cancer cell lines can be improved significantly in combination with UVA exposure. However, the phototoxic property of coralyne was much higher than that of the other two alkaloids. The combination of coralyne and UVA (designated as CUVA) induced oxygen-independent cytotoxicity in the human lung cancer A549 cells by producing more lethal DNA double-strand breaks, and the effect was mediated via the replication machinery. In comparison, the berberine-induced phototoxicity to the A549 cells was mediated by reactive oxygen species generation, mitochondrial membrane permeabilisation and caspase-9/caspase-3 activation.
在这项研究中，我们证明了小檗碱类如苦参碱、黄连素和小檗碱等对几种人类癌细胞系的细胞毒性可以通过与 UVA 暴露联合使用得到显著提高。然而，苦参碱的光毒性比其他两种生物碱高得多。苦参碱与 UVA 的联合作用（称为 CUVA）通过产生更多致死性的 DNA 双链断裂，在人肺癌 A549 细胞中诱导了与氧无关的细胞毒性，其作用是通过复制机制介导的。相比之下，黄连素诱导的 A549 细胞的光毒性是通过活性氧的产生、线粒体膜通透性和 caspase-9/caspase-3 激活来介导的。
