钴-60 放疗增敏 A549 细胞通过上调 CDKN1A 表达来减轻细胞周期 G2/M 期阻滞。

Coralyne Radiosensitizes A549 Cells by Upregulation of CDKN1A Expression to Attenuate Radiation Induced G2/M Block of the Cell Cycle.

机构信息

Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, 25-406 Kielce, Poland.

Centre for Radiobiology and Biological Dosimetry, Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.

出版信息

Int J Mol Sci. 2021 May 28;22(11):5791. doi: 10.3390/ijms22115791.

DOI:10.3390/ijms22115791

PMID:34071406

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8198675/

Abstract

Coralyne is a synthetic analog of berberine related to protoberberine-isoquinoline alkaloids. Isoquinoline derivatives and analogs are renowned as potent radiosensitizers with potential medical application. In the present study, we investigated the effect of coralyne on the cell death, cytoskeletal changes and cell cycle progression of irradiated A549 cells. A clonogenic assay revealed that coralyne pretreatment decreased the viability of A549 cells in a time- and dose-dependent manner. Moreover, exposure to coralyne and ionizing radiation (IR) markedly altered the filamentous actin cytoskeletal architecture and integrin-β binding sites of A549 cells. Treatment with 1-25 µM coralyne in combination with 2 Gy of IR significantly reduced the percentage of cells in G2/M phase compared with 2 Gy IR alone. These results indicate that coralyne is a potent radiosensitizing agent that may find an application in medicine.

摘要

科拉琳是一种与原小檗碱相关的小檗碱合成类似物，属于异喹啉生物碱。异喹啉衍生物及其类似物是众所周知的强效放射增敏剂，具有潜在的医学应用价值。在本研究中，我们研究了科拉琳对受照射的 A549 细胞细胞死亡、细胞骨架变化和细胞周期进程的影响。集落形成实验显示，科拉琳预处理以时间和剂量依赖的方式降低了 A549 细胞的活力。此外，暴露于科拉琳和电离辐射（IR）显著改变了 A549 细胞的丝状肌动蛋白细胞骨架结构和整合素-β结合位点。与单独接受 2 Gy IR 相比，用 1-25 μM 科拉琳联合 2 Gy IR 处理可显著降低 G2/M 期细胞的百分比。这些结果表明，科拉琳是一种有效的放射增敏剂，可能在医学上有应用。