Laboratory for Marine Drugs and Bioproducts, National Laboratory for Marine Science and Technology, Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China.
Biosensors (Basel). 2023 Apr 19;13(4):489. doi: 10.3390/bios13040489.
The effective and accurate detection of the anticancer drug coralyne (COR) is highly significant for drug quality control, medication safety and good health. Although various COR sensors have been reported in recent years, previous ones can only exhibit single-signal output (turn ON or turn OFF) with poor reliability and anti-interference ability. Therefore, exploring novel platform with dual-signal response for COR detection is urgently needed. Herein, we reported the first ratiometric fluorescent platform for highly sensitive and selective COR detection by integrating G-quadruplex (G4) and Pyrene (Py) as signal probes and harnessing A-COR-A interaction. In the absence of COR, the platform shows a low fluorescence signal of PPIX (F) and a high one of Py monomer (F). With the addition of COR, two delicately designed poly-A ssDNAs will hybridize with each other via A-COR-A coordination to form complete G4, yielding the increased fluorescence signal of PPIX and the decreased one of Py due to the formation of Py excimer. Based on the above mechanism, we constructed a simple and efficient sensor that could realize the ratiometric fluorescent detection of COR with high sensitivity and selectivity. A linear relationship between F/F and COR's concentration is obtained in the range from 1 nM to 8 μM. And the limit of detection of COR could reach to as low as 0.63 nM without any amplification, which is much lower than that of most COR sensors reported so far. Notably, the logical analysis of COR can be carried out under the control of a "YES-NOT" contrary logic pair, enabling the smart dual-channel response with an adequate S/N ratio and improved reliability and anti-interference ability. Moreover, this system also presents satisfactory performance in fetal bovine serum (FBS) samples.
抗癌药物考来啉 (COR) 的有效和准确检测对于药物质量控制、用药安全和身体健康至关重要。尽管近年来已经报道了各种 COR 传感器，但以前的传感器只能进行单一信号输出（开或关），可靠性和抗干扰能力较差。因此，迫切需要探索具有双信号响应的新型平台来检测 COR。在此，我们报道了第一个比率荧光平台，用于通过整合 G-四链体 (G4) 和芘 (Py) 作为信号探针，并利用 A-COR-A 相互作用，来进行高灵敏度和选择性 COR 检测。在没有 COR 的情况下，该平台显示出 PPIX（F）的低荧光信号和 Py 单体（F）的高荧光信号。随着 COR 的加入，两个精心设计的 Poly-A ssDNA 将通过 A-COR-A 配位相互杂交形成完整的 G4，由于 Py 激基复合物的形成，导致 PPIX 的荧光信号增加和 Py 的荧光信号减少。基于上述机制，我们构建了一个简单而高效的传感器，可以实现 COR 的比率荧光检测，具有高灵敏度和选择性。在 1 nM 至 8 μM 的范围内，F/F 与 COR 浓度之间存在线性关系。并且 COR 的检测限可以低至 0.63 nM，无需任何放大，这远低于迄今为止报道的大多数 COR 传感器。值得注意的是，COR 的逻辑分析可以在“是-否”相反逻辑对的控制下进行，从而实现具有足够信噪比和提高可靠性和抗干扰能力的智能双通道响应。此外，该系统在胎牛血清 (FBS) 样品中也表现出令人满意的性能。
