Polkowska J, Dubois M P, Jutisz M
Reprod Nutr Dev (1980). 1987;27(3):627-39. doi: 10.1051/rnd:19870502.
Using the immunoperoxidase method, neurons containing luteinizing hormone-releasing hormone (LHRH) and somatostatin (SRIF) were identified in the hypothalamus of growing ewe lambs between 3 days and 16 weeks of age. Both peptidergic neuronal systems were well developed in the early postnatal period. The centres producing LHRH were found to be situated in the anterior region of the hypothalamus and preoptic area. Immunoreactive (ir) LHRH perikarya in growing lambs were readily stained by the various anti-LHRH antibodies used and they were generally more numerous in young animals than in foetuses and adults. The concentration of irLHRH material stored in the median eminence (ME) increased with lamb age, reaching a maximum around 12 weeks of age. The centre producing somatostatin, situated in the anterior periventricular area of the hypothalamus, did not develop before the postpartum period. The irSRIF material in the ME was greatly depleted during the first neonatal days but regained its former level by 8 weeks of age. From 10 to 16 weeks of age, these stores in the ME were again depleted. The results show that the two neuronal systems investigated developed in a different way in the growing lamb. The secretory activity of these systems changed during subsequent periods of growth. The importance of these events in the processes of body growth and reproductive system maturation are still to be determined.
采用免疫过氧化物酶法，在3日龄至16周龄的生长中的母羊下丘脑内鉴定出含有促黄体生成激素释放激素（LHRH）和生长抑素（SRIF）的神经元。在出生后早期，这两种肽能神经元系统均发育良好。发现产生LHRH的中枢位于下丘脑前部区域和视前区。生长中的羔羊体内的免疫反应性（ir）LHRH核周体很容易被所用的各种抗LHRH抗体染色，并且它们在幼龄动物中通常比在胎儿和成年动物中更多。储存在正中隆起（ME）中的irLHRH物质浓度随羔羊年龄增加，在约12周龄时达到最高值。产生生长抑素的中枢位于下丘脑室周前区，在产后时期之前未发育。ME中的irSRIF物质在出生后的头几天大量减少，但在8周龄时恢复到先前水平。从10至16周龄，ME中的这些储存物再次减少。结果表明，所研究的这两种神经元系统在生长中的羔羊体内以不同方式发育。这些系统的分泌活性在随后的生长阶段发生了变化。这些事件在身体生长和生殖系统成熟过程中的重要性仍有待确定。
