Takahashi S, Ono R, Nomura K, Kawashima S
Zoological Institute, Faculty of Science, Hiroshima University, Japan.
Anat Embryol (Berl). 1988;178(6):475-80. doi: 10.1007/BF00305034.
Luteinizing hormone-releasing hormone (LHRH) neurons were immunohistochemically studied in rats of both sexes at peripubertal ages. The number of immunoreactive LHRH neurons (irLHRH neurons) was counted in the brain region from the level of the septum-preoptic area to the level of the rostral part of the infundibulum in colchicine-treated male and female rats at 30 and 60 days of age. At 30 days, irLHRH neurons were more numerous in male rats than females. At 60 days, the number of irLHRH neurons in female rats increased to the level of male rats of the same age. In non-colchicine-treated rats, the count of irLHRH neuron was quite low. The difference in the number of irLHRH neurons between colchicine-treated and non-treated rats may be regarded as the activity of LHRH system. The difference in the number of irLHRH neurons was larger in male rats than in female rats at 30 days of age. On the contrary, at 60 days of age, the difference was larger in females than in males. LHRH contents were measured in the preoptic-anterior hypothalamic area (POA-AH), where LHRH neuronal perikarya are mainly located, and in the mid-hypothalamic area. LHRH content of the POA-AH in male rats at 60 days of age was not significantly different from that at 30 days of age. While, LHRH content in the POA-AH was greater in 60-day-old female rats at proestrous morning than that in 30-day-old females. At 30 days of age, male rats tended to contain more LHRH in the POA-AH than female rats.(ABSTRACT TRUNCATED AT 250 WORDS)
对青春期前后的雌雄大鼠进行了促黄体生成素释放激素（LHRH）神经元的免疫组织化学研究。在30日龄和60日龄经秋水仙碱处理的雄性和雌性大鼠中，对从隔区-视前区水平到漏斗前部水平的脑区中免疫反应性LHRH神经元（irLHRH神经元）的数量进行了计数。30日龄时，雄性大鼠中的irLHRH神经元比雌性大鼠更多。60日龄时，雌性大鼠中irLHRH神经元的数量增加到与同龄雄性大鼠相同的水平。在未经秋水仙碱处理的大鼠中，irLHRH神经元的计数相当低。经秋水仙碱处理和未处理的大鼠之间irLHRH神经元数量的差异可被视为LHRH系统的活性。30日龄时，雄性大鼠中irLHRH神经元数量的差异比雌性大鼠更大。相反，60日龄时，雌性大鼠中的差异比雄性大鼠更大。在视前-下丘脑前部区域（POA-AH，LHRH神经元胞体主要位于此处）和下丘脑中部区域测量了LHRH含量。60日龄雄性大鼠POA-AH中的LHRH含量与30日龄时无显著差异。而在动情前期早晨，60日龄雌性大鼠POA-AH中的LHRH含量高于30日龄雌性大鼠。30日龄时，雄性大鼠POA-AH中的LHRH含量往往比雌性大鼠更多。（摘要截断于250字）
