非酒精性脂肪性肝病患者中瘦人、超重和肥胖者的游离脂肪酸谱：一项病例对照研究。
Free fatty acids profile among lean, overweight and obese non-alcoholic fatty liver disease patients: a case - control study.
机构信息
Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, 157 Baojian Street, Nangang District, Harbin, Heilongjiang Province, 150081, China.
STD & AIDS Center, Harbin Center for Disease Control and Prevention, Harbin, Heilongjiang Province, 150056, China.
出版信息
Lipids Health Dis. 2017 Sep 4;16(1):165. doi: 10.1186/s12944-017-0551-1.
BACKGROUND
Non-alcoholic fatty liver disease (NAFLD) given its association with obesity and diabetes may perhaps exert distinct free fatty acids (FFA) pattern, but the understanding of this phenomenon is limited. To this effect, we evaluated FFA profiles among healthy subjects and NAFLD patients stratified by body weight, to identify FFA valuable for early diagnosis of NAFLD.
METHODS
Serum FFA profiles of healthy and NAFLD (lean, overweight and obese) subjects was determined using gas chromatography-mass spectrometry (GC-MS) and distinctions in FFA patterns were evaluated using one-way ANOVA while Receiver operating characteristics (ROC) and logistic regression models were used to explore FFA significant for diagnosing NAFLD.
RESULTS
NAFLD patients presented significantly higher (P < 0.05) serum FFA profiles compared to healthy controls (HC). While total FFA profiles were insignificantly different between lean (2093.33 ± 558.11 μg/ml) and overweight (2420.81 ± 555.18 μg/ml) NAFLD patients, obese NAFLD (2739.01 ± 810.35 μg/ml) presented most significantly elevated (P < 0.05) total FFA profiles compared with HC. Of the four FFA; myristic acid (14:0), palmitoleic acid (16:1), γ-linolenic acid (γ-18:3) and cis-7,10,13,16,19-docosapentaenoic acid (22:5), selected in ROC analysis given their high Youden's index and AUC, only 14:0; 5.58(1.37, 22.76) and 16:1; 4.36(1.34, 14.13) had statistical significant odd ratios.
CONCLUSION
Our findings suggest 14:0 and 16:1 are promising for early diagnosis of NAFLD.
背景
非酒精性脂肪性肝病（NAFLD）与肥胖和糖尿病有关，其游离脂肪酸（FFA）模式可能有所不同，但对这一现象的了解有限。为此，我们评估了按体重分层的健康受试者和 NAFLD 患者的 FFA 谱，以确定对 NAFLD 早期诊断有价值的 FFA。
方法
采用气相色谱-质谱联用（GC-MS）法测定健康受试者和 NAFLD（瘦、超重和肥胖）患者的血清 FFA 谱，采用单因素方差分析评估 FFA 谱的差异，采用受试者工作特征（ROC）和逻辑回归模型探讨对诊断 NAFLD 有意义的 FFA。
结果
与健康对照组（HC）相比，NAFLD 患者的血清 FFA 谱显著升高（P<0.05）。虽然瘦型（2093.33±558.11μg/ml）和超重型（2420.81±555.18μg/ml）NAFLD 患者的总 FFA 谱无显著差异，但肥胖型 NAFLD（2739.01±810.35μg/ml）与 HC 相比，总 FFA 谱显著升高（P<0.05）。在 4 种 FFA 中，选择 14:0、16:1、γ-亚麻酸（γ-18:3）和顺式-7,10,13,16,19-二十二碳五烯酸（22:5）进行 ROC 分析，因为它们具有较高的 Youden 指数和 AUC，只有 14:0（5.58[1.37, 22.76]）和 16:1（4.36[1.34, 14.13]）具有统计学显著的优势比。
结论
我们的研究结果表明，14:0 和 16:1 对 NAFLD 的早期诊断具有较大潜力。
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