Krajčíková Daniela, Forgáč Vladimír, Szabo Adam, Barák Imrich
Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovakia.
Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovakia.
Microbiol Res. 2017 Nov;204:72-80. doi: 10.1016/j.micres.2017.08.004. Epub 2017 Aug 8.
Bacillus subtilis spores, representatives of an exceptionally resistant dormant cell type, are encircled by a thick proteinaceous layer called the spore coat. More than 80 proteins assemble into four distinct coat layers: a basement layer, an inner coat, an outer coat and a crust. As the spore develops inside the mother cell, spore coat proteins synthesized in the cytoplasm are gradually deposited onto the prespore surface. A small set of morphogenetic proteins necessary for spore coat morphogenesis are thought to form a scaffold to which the rest of the coat proteins are attached. Extensive localization and proteomic studies using wild type and mutant spores have revealed the arrangement of individual proteins within the spore coat layers. In this study we examined the interactions between the proteins localized to the outer coat and crust using a bacterial two hybrid system. These two layers are composed of at least 25 components. Self-interactions were observed for most proteins and numerous novel interactions were identified. The most interesting contacts are those made with the morphogenetic proteins CotE, CotY and CotZ; these could serve as a basis for understanding the specific roles of particular proteins in spore coat morphogenesis.
枯草芽孢杆菌孢子是一种具有极强抗性的休眠细胞类型的代表，其被一层称为芽孢衣的厚蛋白质层所包围。80多种蛋白质组装成四个不同的芽孢衣层：基层、内层、外层和外壳。随着孢子在母细胞内发育，在细胞质中合成的芽孢衣蛋白会逐渐沉积到前孢子表面。一小部分芽孢衣形态发生所必需的形态发生蛋白被认为形成了一个支架，其余的芽孢衣蛋白附着在该支架上。使用野生型和突变型孢子进行的广泛定位和蛋白质组学研究揭示了各个蛋白质在芽孢衣层内的排列。在本研究中，我们使用细菌双杂交系统研究了定位于外层和外壳的蛋白质之间的相互作用。这两层至少由25种成分组成。大多数蛋白质都观察到了自相互作用，并鉴定出了许多新的相互作用。最有趣的相互作用是与形态发生蛋白CotE、CotY和CotZ的相互作用；这些相互作用可为理解特定蛋白质在芽孢衣形态发生中的具体作用提供基础。
