State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Anal Chim Acta. 2017 Sep 15;986:161-170. doi: 10.1016/j.aca.2017.07.030. Epub 2017 Jul 14.
The advantages of spore-based microspheres include high monodispersity, presence of different functional groups (carboxylic, amino, and hydroxyl groups), facile industrial-scale preparation by using cell cultures via fermentation in a potentially cost effective and environment friendly manner, and high uniformity. We developed a novel spore@Fe microsphere for specific capture of phosphoproteins. Caseins (α-casein and β-casein) as phosphoproteins were used to evaluate binding capacity and enrichment factor. The spore@Fe microspheres demonstrate high binding capacity and selectivity for phosphoproteins (1983 and 1818 mg g for α-casein and β-casein, respectively). A mixture of bovine serum albumin and β-casein at 100:1 ratio displayed an enrichment factor higher than 173-fold, which can nearly be considered "purification" of phosphoproteins. The proposed method is a promising technique in developing more selective, rapid, low cost, and high-throughput platforms for phosphoprotein enrichment, and it presents potential application in investigation of protein functions and in personalized diagnostic tests.
基于孢子的微球的优点包括高单分散性、存在不同的官能团（羧基、氨基和羟基）、通过发酵在细胞培养物中以潜在具有成本效益和环保的方式进行易于工业化规模制备，以及高度均一性。我们开发了一种新型的孢子@Fe 微球，用于特定捕获磷酸化蛋白。酪蛋白（α-酪蛋白和 β-酪蛋白）作为磷酸化蛋白用于评估结合能力和富集因子。孢子@Fe 微球对磷酸化蛋白具有高结合能力和选择性（α-酪蛋白和 β-酪蛋白的结合能力分别为 1983 和 1818 mg/g）。牛血清白蛋白和 β-酪蛋白的混合物以 100:1 的比例显示出高于 173 倍的富集因子，几乎可以被认为是磷酸化蛋白的“纯化”。该方法是开发更具选择性、快速、低成本和高通量的磷酸化蛋白富集平台的有前途的技术，并且在蛋白质功能研究和个性化诊断测试中具有潜在的应用。
