Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
College of Life and Health Sciences, Northeastern University, Shenyang, 110169, China.
Mikrochim Acta. 2018 Nov 19;185(12):553. doi: 10.1007/s00604-018-3095-9.
A sorbent for selective extraction of phosphoproteins was obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit an adsorption capacity of 981 mg g for β-casein due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups. The results of LC-MS and SDS-PAGE analysis show that the CeEGO composites can be applied to the extraction of phosphoproteins from protein mixture, and as little as 50 μg mL of the phosphoprotein β-casein can be detected by SDS-PAGE. It was also applied to the extraction of β-casein from spiked biological samples such as drinking milk, whole blood and swine heart tissue extract. Graphical abstract An efficient sorbent is obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit highly selective capture capacity towards phosphoproteins due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups.
通过将 Ce(IV)取代的多金属氧酸盐固定在乙二胺功能化的氧化石墨烯（CeEGO）上，获得了用于选择性提取磷酸化蛋白的吸附剂。由于 Ce(IV)和磷酸基团之间的金属亲和力相互作用以及多金属氧酸盐骨架和磷酸基团之间的π堆积相互作用的协同效应，所得复合材料对 β-酪蛋白表现出 981mg/g 的吸附容量。LC-MS 和 SDS-PAGE 分析结果表明，CeEGO 复合材料可用于从蛋白质混合物中提取磷酸化蛋白，通过 SDS-PAGE 可检测到低至 50μg/mL 的磷酸化蛋白 β-酪蛋白。还将其应用于从添加的生物样品如饮用奶、全血和猪心组织提取物中提取 β-酪蛋白。
