肾提取物可逆转单侧肾切除术后的代偿性细胞增殖。

Kidney extract reversibly inhibits compensatory cell proliferation after unilateral nephrectomy.

作者信息

Skraastad O

出版信息

Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;53(2):102-6. doi: 10.1007/BF02890231.

Abstract

Unilateral nephrectomy (uNX) in mice is followed by a transitory increase in cell proliferation in the remaining kidney. To examine whether this response could be related to a negative feedback control of kidney epithelial cell renewal, water extracts were made of kidney homogenate. Five mg freeze-dried extract was injected 18 h post-operatively, and the animals were sacrificed at intervals during the following 54 h. The mitotic rate and the incorporation of tritiated thymidine (3H-TdR) into DNA were measured in the remaining kidney. The results show that the kidney extract reduces both the mitotic rate and the incorporation of 3H-TdR into DNA. In the tubular epithelium in the kidney, the strongest inhibitory effect was found by injecting the extract at 18 or 39 h postoperatively.

摘要

小鼠单侧肾切除术后，剩余肾脏中的细胞增殖会出现短暂增加。为了研究这种反应是否与肾上皮细胞更新的负反馈控制有关，制备了肾脏匀浆的水提取物。术后18小时注射5毫克冻干提取物，并在接下来的54小时内每隔一段时间处死动物。测定剩余肾脏中的有丝分裂率以及氚标记胸腺嘧啶核苷（3H-TdR）掺入DNA的情况。结果表明，肾脏提取物可降低有丝分裂率以及3H-TdR掺入DNA的量。在肾脏的肾小管上皮中，术后18小时或39小时注射提取物时发现抑制作用最强。