Cimpean Anca Maria, Tarlui Valeria, Cumpănaş Alin Adrian, Bolintineanu Sorin, Cumpănaş Andrei, Raica Marius
Department of Microscopic Morphology/Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy Timisoara, Timisoara, Romania
Department of Microscopic Morphology/Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy Timisoara, Timisoara, Romania.
Anticancer Res. 2017 Sep;37(9):4935-4942. doi: 10.21873/anticanres.11903.
BACKGROUND/AIM: To assess the concordance between IHC and FISH and its influence on tumor type, grade and lympho-vascular invasion (LVI).
HER2 immunohistochemistry (IHC) to 45 cases of bladder carcinoma, followed by fluorescent in situ hybridization (FISH) were applied.
31.12% cases were IHC positive. Less than 35% of HER2-negative cases presented LVI and this percent increased to 54.54% for +1 HER2 cases. LVI increases up to 57.14% for +2 HER2 cases and slightly decreased for +3 HER2 cases to 42.85%. IHC/FISH concordance was found for 73.34% cases but 31.57% were previously negative and 36.36% scored as +1 by IHC had gene amplification as shown by FISH analysis. T3 was correlated with HER2-IHC (p=0.05) and HER2-FISH (p=0.01).
Improved HER2 assessement is needed for urothelial carcinomas. HER2-IHC scored as 0-2 should be validated by and reclassified according to FISH analysis.
背景/目的：评估免疫组化（IHC）与荧光原位杂交（FISH）之间的一致性及其对肿瘤类型、分级和淋巴管浸润（LVI）的影响。
对45例膀胱癌进行HER2免疫组化检测，随后进行荧光原位杂交检测。
31.12%的病例IHC呈阳性。HER2阴性病例中LVI的比例低于35%，HER2 +1病例中这一比例增至54.54%。HER2 +2病例中LVI增至57.14%，HER2 +3病例中略降至42.85%。73.34%的病例发现IHC/FISH结果一致，但FISH分析显示，31.57%之前为阴性，36.36% IHC评分为+1的病例存在基因扩增。T3与HER2-IHC相关（p = 0.05），与HER2-FISH相关（p = 0.01）。
尿路上皮癌需要改进HER2评估。IHC评分为0-2的HER2应通过FISH分析进行验证并重新分类。
